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beta Actin 抗体 (Middle Region)

Name: beta Actin 抗体 (Middle Region)
SKU: ABIN2778644
Availability: OutOfStock

This anti-beta Actin antibody is a 兔多克隆 antibody detecting beta Actin in WB 和 IHC. Suitable for 人, 小鼠, 大鼠, Pig, Cow, 绵羊, 斑马鱼, 马和山羊. This Primary Antibody has been cited in 2+ publications.

WB Suggested Anti-ACTB Antibody Positive Control: Lane 1: 341 µg mouse 3T3 ECM extract + blocking peptide, Lane 2: 041 µg mouse 3T3 ECM extract, Lane 3: 041 µg Echinococcus granulosus extract Primary Antibody Dilution: 1:000Secondary Antibody: Anti-rabbit-HRP Secondry Antibody Dilution: 1:00,000Submitted by: Anonymous (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5. (beta Actin 抗体 (Middle Region))

Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences (beta Actin 抗体 (Middle Region))

IHC

Sample type: 1-8. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Adult Placenta Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTBM Sample Type: Fetal Lung lysates Antibody Dilution: 1ug/ml (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: MCF7 Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Fetal Lung Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

WB Suggested Anti-ACTB Antibody Titration: 0. (beta Actin 抗体 (Middle Region))

Host: Mouse Target Name: ACTG1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Hela Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Human HepG2 Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions : ACTB: Red DAPI:Blue Gene Name : ACTB Submitted by : Dr. (beta Actin 抗体 (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5. (beta Actin 抗体 (Middle Region))

IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml. (beta Actin 抗体 (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: Jurkat Antibody Dilution: 1. (beta Actin 抗体 (Middle Region))

Rabbit Anti-ACTB Antibody Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (beta Actin 抗体 (Middle Region))

IHC

20 images

(2 references)

产品编号 ABIN2778644

发货至: 中国

Datasheet as PDF

Quick Overview for beta Actin 抗体 (Middle Region) (ABIN2778644)

抗原

See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))

适用

人, 小鼠, 大鼠, Pig, Cow, 绵羊, 斑马鱼, 马, 山羊

宿主

兔

克隆类型

多克隆

标记

This beta Actin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product ACTB Primary Antibody

抗原表位
41

17

16

16

8

7

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Middle Region

序列

TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK

预测反应

Cow: 100%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

产品特性

This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the middle region of Human ACTB
anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB 适用: 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Pig, Cow, 绵羊, Hamster, 马, 豚鼠, 山羊, Cat, Avian, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 375 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Phungphong, Kijtawornrat, Wattanapermpool, Bupha-Intr: "Regular exercise modulates cardiac mast cell activation in ovariectomized rats." in: The journal of physiological sciences : JPS, Vol. 66, Issue 2, pp. 165-73, (2016) (PubMed).

Rinne, Clements, Lamme, Duijf, Bolat, Meijer, Scheffer, Rosser, Tan, McGrath, Schalkwijk, Brunner, Zhou, van Bokhoven: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, (2008) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

ACTB

背景

This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Alias Symbols: BRWS1, PS1TP5BP1
Protein Interaction Partner: FUS, DSTN, CAP2, CFL2, CFL1, ACTG1, ACTB, PA2G4, EHHADH, WDYHV1, HDAC6, ISG15, TP53, CEP250, TUBGCP2, TUBGCP3, VCP, UBC, TUBG1, SUMO2, LGALS3BP, AMOT, NEDD1, HAUS1, IVNS1ABP, STAU1, MDM2, CORO7, LGR4, ASB12, ASB9, ZBTB1, ERG, RPA3, RPA2, RPA1, CDKN2A, PAR
Protein Size: 375

分子量

42 kDa

基因ID

60

NCBI登录号

NM_001101, NP_001092

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction