NEDD4-2 抗体 (Middle Region)
(1 reference)Quick Overview for NEDD4-2 抗体 (Middle Region) (ABIN2774731)
抗原
See all NEDD4-2 (NEDD4L) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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序列
- TVTLSAPLEG AKDSPVRRAV KDTLSNPQSP QPSPYNSPKP QHKVTQSFLP
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预测反应
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
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产品特性
- This is a rabbit polyclonal antibody against NEDD4L. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L
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anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 1-100) antibody
NEDD4L 适用: 人 ELISA, WB, IP 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 836-955) antibodyNEDD4L 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) antibodyNEDD4L 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 289-494) antibodyNEDD4L 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) antibodyNEDD4L 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (pSer448) antibodyNEDD4L 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 1-100) antibodyNEDD4L 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 275-325) antibodyNEDD4L 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Pig WB, IHC, IF, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 225-275) antibodyNEDD4L 适用: 人, 小鼠, Cow, Pig, 兔 WB, IHC, IF, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E3 ubiquitin-protein ligase NEDD4-like (NEDD4L) (AA 1-911) antibodyNEDD4L 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 955 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, (2008) (PubMed).
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: "Nedd4-2 isoforms ubiquitinate individual epithelial sodium channel subunits and reduce surface expression and function of the epithelial sodium channel." in: American journal of physiology. Renal physiology, Vol. 294, Issue 5, pp. F1157-65, (2008) (PubMed).
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- NEDD4-2 (NEDD4L) (E3 ubiquitin-protein ligase NEDD4-like (NEDD4L))
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别名
- NEDD4L
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背景
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NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
Alias Symbols: FLJ33870, KIAA0439, RSP5, hNedd4-2, NEDD4-2, NEDD4.2, hNEDD4-2
Protein Interaction Partner: NEDD4L, DAZAP2, UBE2D1, PTCH1, WNK3, TNK2, SNCA, SLC9A3, SMAD6, KCNH2, HCN1, SMAD3, UBE2N, SNA3, NOTCH1, UBE2D3, UBC, SGK1, KCNQ1, SGK3, GORAB, MED28, FBXL15, vpr, RNF11, AATF, YWHAQ, LAPTM5, SCNN1B, SMAD2, ARRDC3, ARRDC4, UBE2L3, UBE2D2, SMAD7, UBE2K, UB
Protein Size: 955 -
分子量
- 110 kDa
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基因ID
- 23327
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NCBI登录号
- NM_015277, NP_056092
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途径
- Negative Regulation of Transporter Activity
抗原
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