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L-Selectin 抗体

Name: L-Selectin 抗体
Brand: antibodies-online
SKU: ABIN2749031
Availability: OutOfStock

Cited in 6+ publications. This 小鼠单克隆 anti-L-Selectin antibody (Clone DREG56) specifically detects L-Selectin in FACS, WB, IP, Func 和 IHC (fro). The antibody is reactive with 人 samples.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (low endotoxin, concentration in sample 1 μg/mL) GAM APC. (L-Selectin 抗体)

FACS

Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (low endotoxin, concentration in sample 1 μg/mL) GAM APC. (L-Selectin 抗体)

FACS

2 images

(6 references)

产品编号 ABIN2749031

发货至: 中国

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Quick Overview for L-Selectin 抗体 (ABIN2749031)

抗原

See all L-Selectin (SELL) 抗体

L-Selectin (SELL) (Selectin L (SELL))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This L-Selectin antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

DREG56

Discover our top product SELL Primary Antibody

原理

Anti-Hu CD62L Purified Low Endotoxin

特异性

The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells.

交叉反应（详细）

Human

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

内毒素水平

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

免疫原

PMA-activated human peripheral blood leukocytes

亚型

IgG1
anti-Selectin L (SELL) (AA 301-372) antibody
SELL 适用: 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Selectin L (SELL) (AA 1-100) antibody
SELL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Selectin L (SELL) (AA 83-186) antibody
SELL 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 8C8B7 unconjugated

anti-Selectin L (SELL) (AA 109-346) antibody
SELL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Selectin L (SELL) antibody
SELL 适用: 人, Cow FACS, IP, ICC 宿主: 小鼠 Monoclonal IVA94 unconjugated

anti-Selectin L (SELL) antibody
SELL 适用: 小鼠 FACS, IP, IHC (fro), ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated

anti-Selectin L (SELL) antibody
SELL 适用: 小鼠 FACS, IP, Func, IHC (fro), ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated

anti-Selectin L (SELL) antibody
SELL 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal DREG56 unconjugated

anti-Selectin L (SELL) antibody
GMP Grade SELL 适用: 人, Baboon FACS 宿主: 小鼠 Monoclonal DREG56 unconjugated

anti-Selectin L (SELL) antibody (PE)
GMP Grade SELL 适用: 人, Baboon FACS 宿主: 小鼠 Monoclonal DREG56 PE

应用备注

Functional application: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV.
Flow cytometry: Recommended dilution: 2 μg/mL.
Western blotting: Non-reducing conditions.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Tu, Mao, Zheng, Liu, Chiu, Qin, Chan, Lam, Guan, Zhang, Guan, Yuen, Peiris, Lau: "Cytotoxic T lymphocytes established by seasonal human influenza cross-react against 2009 pandemic H1N1 influenza virus." in: Journal of virology, Vol. 84, Issue 13, pp. 6527-35, (2010) (PubMed).

Killock, Parsons, Zarrouk, Ameer-Beg, Ridley, Haskard, Zvelebil, Ivetic: "In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin." in: The Journal of biological chemistry, Vol. 284, Issue 13, pp. 8833-45, (2009) (PubMed).

Xu, Chen, Shang, Cui, Luo, Chen, Ba, Zeng: "Critical role of Lck in L-selectin signaling induced by sulfatides engagement." in: Journal of leukocyte biology, Vol. 84, Issue 4, pp. 1192-201, (2008) (PubMed).

Jutila, Kurk, Jackiw, Knibbs, Stoolman: "L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1768-73, (2002) (PubMed).

Kishimoto, Warnock, Jutila, Butcher, Lane, Anderson, Smith: "Antibodies against human neutrophil LECAM-1 (LAM-1/Leu-8/DREG-56 antigen) and endothelial cell ELAM-1 inhibit a common CD18-independent adhesion pathway in vitro." in: Blood, Vol. 78, Issue 3, pp. 805-11, (1991) (PubMed).

Kishimoto, Jutila, Butcher: "Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 6, pp. 2244-8, (1990) (PubMed).
抗原

L-Selectin (SELL) (Selectin L (SELL))

别名

CD62L

背景

Selectin L,CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).,L-selectin, LSEL, SELL, LAM-1, LECAM1, LEU8, LNHR, LYAM1, PLNHR

基因ID

6402

UniProt

P14151

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