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NGFR 抗体 (Biotin)

NGFR 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal ANC271-3D7 Biotin
产品编号 ABIN2743244
发货至: 中国
  • 抗原 See all NGFR 抗体
    NGFR (Nerve Growth Factor Receptor (NGFR))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This NGFR antibody is conjugated to Biotin
    应用范围
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    Flow Cytometry (FACS), ELISA
    特异性
    Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.
    纯化方法
    Protein A purified
    免疫原
    Recombinant soluble human CD271.

    Type of Immunogen: Recombinant protein
    克隆位点
    ANC271-3D7
    亚型
    IgG1 kappa
    Top Product
    Discover our top product NGFR Primary Antibody
  • 应用备注
    Approved: ELISA, Flo

    Usage: Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD271/Biotin at a concentration of 10 μg/mL. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store at 4°C, avoid freeze-thaw cycles.
  • 抗原
    NGFR (Nerve Growth Factor Receptor (NGFR))
    别名
    NGFR / CD271 (NGFR 产品)
    别名
    ngfr antibody, wu:fb38c02 antibody, wu:fc10h01 antibody, CD271 antibody, Gp80-LNGFR antibody, TNFRSF16 antibody, p75(NTR) antibody, p75NTR antibody, LNGFR antibody, RNNGFRR antibody, Tnfrsf16 antibody, p75 antibody, NRH1b antibody, fullback antibody, nrh1 antibody, p75ntr antibody, tnfrsf16 antibody, p75NGFR antibody, nerve growth factor receptor antibody, neurotrophin receptor associated death domain antibody, nerve growth factor receptor S homeolog antibody, nerve growth factor receptor (TNFR superfamily, member 16) antibody, NGFR antibody, nradd antibody, Ngfr antibody, ngfr.S antibody
    背景
    Name/Gene ID: NGFR
    Family: TNF Receptor

    Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16
    基因ID
    4804
    途径
    NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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