CD3 epsilon 抗体 (Biotin)
Quick Overview for CD3 epsilon 抗体 (Biotin) (ABIN2739337)
抗原
See all CD3 epsilon (CD3E) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.
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纯化方法
- Protein G purified
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免疫原
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Mouse BM10-37 T cell membrane prep.
Type of Immunogen: Membrane preparation -
亚型
- IgG
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anti-CD3 epsilon (CD3E) (AA 23-207) antibody
CD3E 适用: 人, 小鼠, 大鼠, 小鸡 WB, IHC, IF, IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) antibodyCD3E 适用: 人, 小鼠, 大鼠 FACS, IF, Func 宿主: 小鼠 Monoclonal RIV9 unconjugated
anti-CD3 epsilon (CD3E) (AA 23-119) antibodyCD3E 适用: 人 FACS, WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal C3e-1931 unconjugated
anti-CD3 epsilon (CD3E) (AA 156-168), (Cytoplasmic Domain) antibodyCD3E 适用: 人, 大鼠, Pig, Cow, 马, 兔, 豚鼠, 小鸡 FACS, WB, IF 宿主: 小鼠 Monoclonal PC3-188A unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (C-Term) antibodyCD3E 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 25-207) antibodyCD3E 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 28-185) antibodyCD3E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD3 epsilon (CD3E) (AA 23-207) antibodyCD3E 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, Flo, IP, WB
Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated 5 minutes with 20 μL of 250 μg/mL Mouse IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.34 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated with 20^l of 0.5 mg/mL unlabeled anti-CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 4°C, avoid freeze-thaw cycles.
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- CD3 epsilon (CD3E)
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别名
- CD3E
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背景
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Name/Gene ID: CD3E
Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon -
基因ID
- 916
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途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
抗原
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