14-3-3 zeta 抗体
(2 references)Quick Overview for 14-3-3 zeta 抗体 (ABIN272122)
抗原
See all 14-3-3 zeta (YWHAZ) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- This antibody detects endogenous levels of 14-3-3 ζ/δ protein. (region surrounding Trp228)
-
交叉反应 （详细）
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
-
纯度
- > 95 % pure by SDS-PAGE
-
-
anti-14-3-3 zeta (YWHAZ) (AA 133-212) antibody
YWHAZ 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 19G7E9 unconjugated
anti-14-3-3 zeta (YWHAZ) (AA 133-212) antibodyYWHAZ 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (AA 51-150) antibodyYWHAZ 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B3 unconjugated
anti-14-3-3 zeta (YWHAZ) (pSer58) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (Center) antibodyYWHAZ 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (pSer58) antibodyYWHAZ 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (AA 200-245) antibodyYWHAZ 适用: 人, 小鼠, 大鼠, Cow, 犬, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (Tyr330) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (pThr232) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 zeta (YWHAZ) (C-Term) antibodyYWHAZ 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1,0 mg/mL
-
缓冲液
- Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- DO NOT FREEZE!
-
储存条件
- 4 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C.
-
-
-
: "miRNA-221 and miRNA-222 induce apoptosis via the KIT/AKT signalling pathway in gastrointestinal stromal tumours." in: Molecular oncology, Vol. 9, Issue 7, pp. 1421-33, (2016) (PubMed).
: "Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo-/- mice." in: Chemico-biological interactions, Vol. 206, Issue 2, pp. 143-52, (2013) (PubMed).
-
: "miRNA-221 and miRNA-222 induce apoptosis via the KIT/AKT signalling pathway in gastrointestinal stromal tumours." in: Molecular oncology, Vol. 9, Issue 7, pp. 1421-33, (2016) (PubMed).
-
- 14-3-3 zeta (YWHAZ)
-
别名
- 14-3-3 Protein zeta/delta
-
背景
- 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAZ
-
分子量
- approx. 28 kDa
-
基因ID
- 7534
-
NCBI登录号
- NP_001129171
-
UniProt
- P63104
-
途径
- Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
抗原
-
