PKC alpha 抗体
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- 抗原 See all PKC alpha (PKCa) 抗体
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PKC alpha antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects endogenous levels of PKC α, β I, II, γ, δ, ε, η, ζ and θ isoforms. (region surrounding Thr495)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- 纯度
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product PKCa Primary Antibody
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anti-Protein Kinase C, alpha (PKCa) (AA 563-672) antibody
PKCa 适用: 人 WB, ELISA, IHC (p), IF, PLA 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-Protein Kinase C, alpha (PKCa) antibodyPKCa 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IP, IF, ICC 宿主: 兔 Monoclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (AA 299-329) antibodyPKCa 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (pThr638) antibodyPKCa 适用: 人, 小鼠 WB, FACS, IHC (p), IF (cc) 宿主: 兔 Monoclonal 4B3 unconjugated
anti-Protein Kinase C, alpha (PKCa) (pThr638) antibodyPKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) antibodyPKCa 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (AA 299-329), (C-Term) antibodyPKCa 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB01012 unconjugated
anti-Protein Kinase C, alpha (PKCa) (C-Term) antibodyPKCa 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (C-Term) antibodyPKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C, alpha (PKCa) (N-Term) antibodyPKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 5000approx. 1: 10000. Western blot: 1: 500approx. 1: 1000. Immunohistochemistry on paraffin sections: 1: 50approx. 1: 200. Immunflourescence: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for up to one month. For long term storage, aliquot and store at -20 °C.
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- 抗原
- PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
- 别名
- PRKCA (PKCa 产品)
- 别名
- pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody
- 背景
- Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involved. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu.Synonyms: Protein kinase C
- 分子量
- approx. 75-88 kDa
- 基因ID
- 5578
- NCBI登录号
- NP_002728
- UniProt
- P17252
- 途径
- WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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