p300 抗体
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Quick Overview for p300 抗体 (ABIN272047)
抗原
See all p300 (EP300) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of p300 protein. (region surrounding Glu83)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-E1A Binding Protein P300 (EP300) (AA 731-830) antibody
EP300 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B1 unconjugated
anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibodyEP300 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-E1A Binding Protein P300 (EP300) antibodyEP300 适用: 人 WB, IP, IF, ICC, ChIP, ChIP-seq 宿主: 小鼠 Monoclonal NM11 unconjugated
anti-E1A Binding Protein P300 (EP300) (AA 1351-1601) antibodyEP300 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-E1A Binding Protein P300 (EP300) (AA 1-158) antibodyEP300 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-E1A Binding Protein P300 (EP300) (AA 2283-2414) antibodyEP300 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-E1A Binding Protein P300 (EP300) (N-Term) antibodyEP300 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E1A Binding Protein P300 (EP300) (AA 2124-2397) antibodyEP300 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-E1A Binding Protein P300 (EP300) (AA 731-831) antibodyEP300 适用: 人 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B1 unconjugated
anti-E1A Binding Protein P300 (EP300) (N-Term) antibodyEP300 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH ~7.2, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- p300 (EP300) (E1A Binding Protein P300 (EP300))
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别名
- EP300 / P300
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背景
- Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A, bind to this element. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 Molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.Synonyms: E1A binding protein p300, E1A-associated protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT
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分子量
- approx. 265.0 kDa
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基因ID
- 2033
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NCBI登录号
- NP_001420
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UniProt
- Q09472
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途径
- p53 Pathway, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
抗原
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