ELISA: 1: 10000approx. 1: 20000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1,0 mg/mL
缓冲液
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
DO NOT FREEZE!
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
MTOR (mTOR)
(Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
Regulatory associated protein of mTOR, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (mTOR), and is essential for mTOR signalling in vivo. Raptor binding to mTOR is critical for mTORcatalysed substrate phosphorylation of 4EBP1. The Raptor-mTOR complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of mTOR kinase activity under nutrient-deprived conditions and is an important component in the mTOR pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR