ARAF 抗体
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北京 101111
Quick Overview for ARAF 抗体 (ABIN271994)
抗原
See all ARAF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of A-RAF protein. (region surrounding Asp297)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 151-250) antibody
ARAF 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3E2 unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibodyARAF 适用: 人, 小鼠 WB, IF (cc), IHC (p) 宿主: 兔 Monoclonal 24D1 unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) antibodyARAF 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (C-Term) antibodyARAF 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 160-310) antibodyARAF 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) antibodyKD Validated ARAF 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB440 unconjugated Recombinant Antibody
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pTyr301), (pTyr302) antibodyARAF 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (pSer299) antibodyARAF 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (Internal Region) antibodyARAF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 309-413) antibodyARAF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
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别名
- ARAF
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背景
- Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.Synonyms: A-Raf proto-oncogene serine/threonine-protein kinase, A-raf-1, ARAF1, PKS, PKS2, Proto-oncogene Pks
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分子量
- approx. 68 kDa
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基因ID
- 369
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NCBI登录号
- NP_001645
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UniProt
- P10398
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途径
- MAPK Pathway, RTK signaling, Hepatitis C
抗原
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