p21 抗体
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北京 101111
Quick Overview for p21 抗体 (ABIN271762)
抗原
See all p21 (CDKN1A) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of p21 Cip1 protein. (region surrounding Gly139)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 65-164) antibody
CDKN1A 适用: 人 WB, IF, ELISA, PLA 宿主: 小鼠 Monoclonal 2F1 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-164) antibodyCDKN1A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-100) antibodyCDKN1A 适用: Synthetic WB, IHC (f) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibodyCDKN1A 适用: 人, Non-Human Primate ELISA, IHC (p), ICC 宿主: 小鼠 Monoclonal WA-1 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 21-100) antibodyCDKN1A 适用: 人, 小鼠, 大鼠, 绵羊 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr145) antibodyCDKN1A 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibodyKD Validated CDKN1A 适用: 人 WB, FACS, IF (p) 宿主: 兔 Monoclonal 25GB3200 unconjugated Recombinant Antibody
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) antibodyCDKN1A 适用: 人 WB, IHC, IF, IHC (f) 宿主: 小鼠 Monoclonal CIP1-823 unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-164) antibodyCDKN1A 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (C-Term) antibodyCDKN1A 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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别名
- CDKN1A / p21WAF1
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背景
- It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. A novel Cdk-interacting protein, designated p21, Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 is designated CAP20.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
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分子量
- approx. 21.0 kDa
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基因ID
- 1026
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NCBI登录号
- NP_000380
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UniProt
- P38936
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途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
抗原
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