CTLA4 抗体
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北京 101111
Quick Overview for CTLA4 抗体 (ABIN7304772)
抗原
See all CTLA4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse monoclonal antibody to CD152
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特异性
- Recognizes human CD152
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产品特性
- Mouse monoclonal antibody to CD152
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纯化方法
- The antibody was purified by affinity chromatography.
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免疫原
- Native purified human CD152.
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亚型
- IgG2a
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anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibody (PE)
CTLA4 适用: 人 FACS 宿主: 小鼠 Monoclonal BNI3 PE
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-135) antibodyCTLA4 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F1 unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-162) antibodyCTLA4 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 9D2E9 unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) antibodyCTLA4 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8B3F8 unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) antibodyCTLA4 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM50 unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibodyCTLA4 适用: 人 FACS, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal BNI3 unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) antibodyCTLA4 适用: 人 FACS, IP, ICC, IHC (fro) 宿主: 小鼠 Monoclonal BNI3 unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) antibodyCTLA4 适用: 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 75-170) antibodyCTLA4 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 52-211) antibodyCTLA4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
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实验流程
- 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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有效期
- 12 months
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- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
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别名
- CD152
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背景
- CD152, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD antigen CD152
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基因ID
- 1493
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UniProt
- P16410
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途径
- Cancer Immune Checkpoints
抗原
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