SLUG 抗体
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北京 101111
Quick Overview for SLUG 抗体 (ABIN2689904)
抗原
See all SLUG (SNAI2) 抗体适用
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克隆类型
标记
应用范围
克隆位点
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产品特性
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The S43-1259 monoclonal antibody specifically recognizes SNAI2, also named Slug, which is a zinc finger transcriptional repressor first recognized for its roles in embryogenesis. SNAI2/Slug and other members of the snail family of transcription factors inhibit the expression of E-cadherin, which plays important roles in cell adhesion and in the maintenance of tissue structure. SNAI2/Slug activation and E-cadherin inhibition induces epithelial-mesenchymal transition (EMT) at several stages of embryonic development and also contributes to the invasiveness of malignancies. Aberrant expression of SNAI2/Slug has also been linked to cancer stem cell formation, cell cycle regulation and apoptosis. SNAI2/Slug is a downstream effector of various signaling pathways, including Akt, Wnt and SCF/ckit signaling. Immunoflourescent, flow cytometric, western blot, and immunohistochemical analysis of SNAI2/Slug in human sarcoma cells. FAR LEFT: RH-30 rhabdomyosarcoma cells (DSMZ, ACC 489) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Perm/Wash™ Buffer (Cat No. 554723) and stained with Purified Mouse Anti-SNAI2/Slug (pseudo-colored green) at 1.2 μg/mL followed by Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies). Nuclear counter -staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software. Permeabilization with BD Phosflow™ Perm Buffer III (Cat. No. 558050) or 0.1 % Triton™ X-100 buffer is also suitable. MIDDLE LEFT: RH-30 cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574) and stained with either Purified Mouse IgG1 κ Isotype Control (Cat No. 554121, dashed line) or Purified Mouse Anti-SNAI2/Slug (solid line), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer. MIDDLE RIGHT: Lysates of RH-30 cells were electrophoresed (SDS-PAGE), transferred to PVDF membranes and then probed with 125, 60, 30, 15 and 7 ng/mL of Purified Mouse Anti-SNAI2/Slug (lanes 1-5) and HRP goat anti-mouse Ig (Cat. No. 554002). FAR RIGHT: Following antigen retrieval with Retrievagen A Buffer (Cat. No. 550524), formalin-fixed paraffin sections of a human fibrosarcoma were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-SNAI2/Slug (right). Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) were used to develop the staining. Original magnification: 40x.
BD Pharmingen™ Purified Mouse Anti-SNAI2/Slug - Purified - Clone S43-1259 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 50 μg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human SNAI2 Recombinant Protein
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亚型
- IgG1 kappa
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anti-Snail Family Zinc Finger 2 (SNAI2) (AA 97-169) antibody
SNAI2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3C12 unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (AA 100-200) antibodySNAI2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 4B6D5 unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (AA 97-169) antibodySNAI2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (AA 98-129) antibodySNAI2 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (AA 89-268) antibodySNAI2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (AA 126-268) antibodySNAI2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) antibodySNAI2 适用: 人 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (AA 69-132) antibodySNAI2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2E10 unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (AA 116-148), (Middle Region) antibodySNAI2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Snail Family Zinc Finger 2 (SNAI2) (Middle Region) antibodySNAI2 适用: 人, 小鼠, 大鼠, Cow, 绵羊, 犬, 马, 兔, 豚鼠, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells." in: Oncogene, Vol. 34, Issue 5, pp. 546-57, (2015) (PubMed).
: "Slug and Sox9 cooperatively determine the mammary stem cell state." in: Cell, Vol. 148, Issue 5, pp. 1015-28, (2012) (PubMed).
: "Expression of Snail protein in tumor-stroma interface." in: Oncogene, Vol. 25, Issue 37, pp. 5134-44, (2006) (PubMed).
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: "Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells." in: Oncogene, Vol. 34, Issue 5, pp. 546-57, (2015) (PubMed).
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- SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))
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别名
- SNAI2/Slug
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背景
- Synonyms: Snai2, SLUG, Slugh, SLUGH
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分子量
- 30-34 kDa
抗原
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