CD73 抗体
(5 references)-
- 抗原 See all CD73 (NT5E) 抗体
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD73 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))
- 品牌
- BD Pharmingen™
- 产品特性
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The 5F/B9 antibody reacts with CD73 or Ecto-5'-nucleotidase (5'-NT), a 68- kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities. 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues. Membrane-bound and/or intracytoplasmic CD73 is found in a variety of organs and tissues, including liver, kidney, adrenal cortex, spleen, muscle, nervous tissue, and heart. The expression of CD73 on mouse leukocytes has been well characterized. In the rat, CD73 has been detected on subsets of peripheral B and T lymphocytes, NK cells, and granulocytes, as well as bone marrow myeloid cells and some CD4+CD8- thymocytes. The 5F/B9 mAb does not inhibit the enzymatic activity of 5'-NT. Although soluble or plate-bound 5F/B9 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with plate-bound anti-rat CD3 mAb G4.18 (Cat. no. 554829). Two-color analysis of the expression of CD73 on rat spleen leukocytes. LEW splenocytes were stained with FITC- conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and either purified mouse IgG1, κ isotype control mAb MOPC-31C Cat. no. 557273, Left panel) or purified mAb 5F/B9 (Right panel), followed by biotinylated anti-mouse IgG1 mAb A85-1 (Cat. no. 553441), then Streptavidin-APC (Cat. no. 554067). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Mouse Anti-Rat CD73 - Purified - Clone 5F/B9 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Ecto-5'-nucleotidase purified from rat renal membranes
- 克隆位点
- 5F-B9
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NT5E Primary Antibody
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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibodyNT5E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, FACS, IHC, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibodyNT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibodyNT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibodyNT5E 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "CD73 expression and fyn-dependent signaling on murine lymphocytes." in: European journal of immunology, Vol. 28, Issue 10, pp. 2981-90, (1998) (PubMed).
: "Ecto-5'-nucleotidase is expressed by pericytes and fibroblasts in the rat heart." in: Histochemistry and cell biology, Vol. 103, Issue 3, pp. 227-36, (1995) (PubMed).
: "5'-nucleotidase in spinal meningeal compartments in the rat. An immuno and enzyme histochemical study." in: Histochemistry, Vol. 98, Issue 2, pp. 135-9, (1992) (PubMed).
: "5'-Nucleotidase: molecular structure and functional aspects." in: The Biochemical journal, Vol. 285 ( Pt 2), pp. 345-65, (1992) (PubMed).
: "Immunolocalization of ecto-5'-nucleotidase in the kidney by a monoclonal antibody." in: Histochemistry, Vol. 95, Issue 2, pp. 165-74, (1991) (PubMed).
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: "CD73 expression and fyn-dependent signaling on murine lymphocytes." in: European journal of immunology, Vol. 28, Issue 10, pp. 2981-90, (1998) (PubMed).
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- 抗原
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- 别名
- CD73 (NT5E 产品)
- 别名
- CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody
- 背景
- Synonyms: Ecto-5'-nucleotidase
- 途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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