(15 references)-
- 抗原 See all CD40 抗体
- CD40
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适用
- 小鼠, 大鼠
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宿主
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Armenian Hamster
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克隆类型
- 单克隆
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标记
- This CD40 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The HM40-3 antibody reacts with CD40, a 40-50- kDa glycoprotein expressed on B lymphocytes and other antigen-presenting cells. The CD40 Molecule has a central role in B-cell growth and differentiation. Furthermore, interactions of CD40 with its ligand, CD154, are involved in the initiation and effector stages of cell-mediated immune responses. CD40 may be involved in the triggering of NK cells and NK-T cells. Soluble HM40-3 antibody stimulates splenic and peritoneal B cells to proliferate in vitro. This antibody also induces spleen B cells to express the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). HM40-3 mAb has been demonstrated to inhibit the binding of soluble CD154 (gp39, CD40 Ligand) to soluble CD40 and to cell-surface CD40. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with similar populations of Lewis, Sprague-Dawley, and LOU16 rat leukocytes. Two-color analysis of the expression of CD40 on mouse spleen cells. BALB/c mouse splenocytes were simultaneously stained with PE Rat Anti-Mouse CD45R/B220 mAb (Cat. No. 553089/553090, both panels) and either Purified Hamster Anti-Mouse CD40 mAb (Cat. No. 553722, Right Panel) or Purified Hamster IgM, λ1 Isotype Standard (Cat. No 553958, Left Panel) followed by FITC Mouse Anti-Armenian Hamster IgM mAb (Cat. No. 554033, both panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
BD Pharmingen™ Purified Hamster Anti-Mouse CD40 - Purified - Clone HM40-3 - Isotype Armenian Hamster IgM, κ - Reactivity Ms, Rat - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- (BALB/c x NZB) F1 Mouse-derived Lymphoma WEHI-231
- 克隆位点
- HM40
- 亚型
- IgM kappa
- Top Product
- Discover our top product CD40 Primary Antibody
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anti-CD40 (CD40) (AA 26-187) antibody
CD40 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IF, StM, DNA Mic 宿主: 小鼠 Monoclonal C40-1605 unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) (AA 29-107) antibodyCD40 适用: 人 FACS, ELISA, IHC, StM, Coat 宿主: 小鼠 Monoclonal C40-2383 unconjugated
anti-CD40 (CD40) (C-Term) antibodyCD40 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) (AA 21-193) antibodyCD40 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 4E1B9 unconjugated
anti-CD40 (CD40) (Extracellular Domain) antibodyCD40 适用: 人 FACS, IHC, St 宿主: 小鼠 Monoclonal 5C3 unconjugated
anti-CD40 (CD40) (AA 1-360) antibodyCD40 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD40 (CD40) antibodyCD40 适用: 人 FACS, IHC, IP, Act 宿主: 兔 Chimeric G28-5 unconjugated Recombinant Antibody
anti-CD40 (CD40) antibody (FITC)CD40 适用: 人 FACS 宿主: 小鼠 Monoclonal HI40a FITC
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 89-94, (2001) (PubMed).
: "A subset of cytolytic dendritic cells in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, (2000) (PubMed).
: "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7058-66, (1999) (PubMed).
: "Enhanced expression of CD80 (B7-1), CD86 (B7-2), and CD40 and their ligands CD28 and CD154 in fulminant hepatic failure." in: The American journal of pathology, Vol. 154, Issue 6, pp. 1711-20, (1999) (PubMed).
: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, (1999) (PubMed).
: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, (1998) (PubMed).
: "A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell." in: Nature, Vol. 393, Issue 6684, pp. 474-8, (1998) (PubMed).
: "CD40-mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus." in: European journal of immunology, Vol. 26, Issue 12, pp. 3061-5, (1997) (PubMed).
: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, (1997) (PubMed).
: "Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells." in: European journal of immunology, Vol. 26, Issue 7, pp. 1451-8, (1996) (PubMed).
: "Activation of thymic B cells by signals of CD40 molecules plus interleukin-10." in: European journal of immunology, Vol. 25, Issue 5, pp. 1244-8, (1995) (PubMed).
: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, (1994) (PubMed).
: "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1849-60, (1994) (PubMed).
: "Hypercross-linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL-4 and anti-CD40." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2821-9, (1994) (PubMed).
: "CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation." in: Immunology today, Vol. 13, Issue 11, pp. 431-3, (1993) (PubMed).
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: "Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 89-94, (2001) (PubMed).
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- 抗原
- CD40
- 别名
- CD40 (CD40 产品)
- 别名
- Bp50 antibody, CDW40 antibody, TNFRSF5 antibody, p50 antibody, AI326936 antibody, GP39 antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TRAP antibody, Tnfrsf5 antibody, TNFSF5 antibody, CD40 molecule antibody, CD40 antigen antibody, CD40 antibody, Cd40 antibody
- 背景
- Synonyms: Bp50, Tnfrsf5, TNR5, TRAP, CD40L receptor, GP39, HIGM1, IMD3, T-BAM
- 途径
- NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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