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CD4 抗体

Name: CD4 抗体
SKU: ABIN2689231
Availability: OutOfStock

This anti-CD4 antibody is a 大鼠单克隆 antibody detecting CD4 in FACS, IP, IHC (fro), BR, IHC (f) 和 IHC (zinc). Suitable for 小鼠. This Primary Antibody has been cited in 9+ publications.

(9 references)

产品编号 ABIN2689231

发货至: 中国

Datasheet as PDF

Quick Overview for CD4 抗体 (ABIN2689231)

抗原

See all CD4 抗体

CD4

适用

小鼠

宿主

大鼠

克隆类型

单克隆

标记

This CD4 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

克隆位点

H129-19

Discover our top product CD4 Primary Antibody

品牌

BD Pharmingen™

产品特性

The H129.19 antibody reacts with the CD4 (L3T4) differentiation antigen expressed on thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. CD4 has also been detected on pluirpotent hematopoietic stem cells, bone marrow myeloid precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 is expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine lck. H129.19 mAb blocks binding of the anti-mouse CD4 mAbs Gk1.5 (Cat. No. 557307/553729) and RM4-5 (Cat. No. 553046/553047), but not RM4-4 (Cat. No. 553055) antibody. mAb H129.19 inhibits various responses of T helper cells to antigenic or mitogenic stimuli. Immunohistochemical staining of CD4 + T lymphocytes. Frozen sections of normal mouse spleen were reacted with H129.19 antibody. CD4+ T lymphocytes can be identified by the intense brown labeling of their cell surface membranes. Amplification 20X.

BD Pharmingen™ Purified Rat Anti-Mouse CD4 - Purified - Clone H129.19 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

A.TH mouse CTL clone A15.1.17

亚型

IgG2a kappa
anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Pig, 兔, Cat, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB, IHC 宿主: 山羊 Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

anti-CD4 (CD4) antibody (PE)
CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

浓度

62.5 μg/mL

缓冲液

Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, (1992) (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

Wu, Scollay, Egerton, Pearse, Spangrude, Shortman: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, (1991) (PubMed).

Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, (1989) (PubMed).

Bierer, Sleckman, Ratnofsky, Burakoff: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, (1989) (PubMed).

Ghobrial, Boublik, Winn, Auchincloss: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, (1989) (PubMed).

Pont, Régnier-Vigouroux, Marchetto, Pierres: "Accessory molecules and T cell activation. II. Antibody binding to L3T4a inhibits Ia-independent mouse T cell proliferation." in: European journal of immunology, Vol. 17, Issue 3, pp. 429-32, (1987) (PubMed).

Naquet, Malissen, Bekkhoucha, Pont, Pierres, Hood, Pierres: "L3T4 but not LFA-1 participates in antigen presentation by Ak-positive L-cell transformants." in: Immunogenetics, Vol. 22, Issue 3, pp. 247-56, (1985) (PubMed).

Pierres, Naquet, Van Agthoven, Bekkhoucha, Denizot, Mishal, Schmitt-Verhulst, Pierres et al.: "A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 6, pp. 2775-82, (1984) (PubMed).
抗原

CD4

别名

CD4

背景

Synonyms: L3T4

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events