(6 references)-
- 抗原 See all CD25 (IL2RA) 抗体
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD25 antibody is conjugated to Biotin
-
应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- BD Pharmingen™
- 产品特性
-
The 7D4 antibody reacts with CD25, the low affinity IL-2 Receptor (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Rα by itself is not a signaling receptor. However, it can combine with IL-2 Receptor β (CD122) and γ (CD132) chains to form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdTsIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+ CD4+ T lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. no. 553068). The 3C7 antibody recognizes an epitope of CD25 which is distinct from those recognized by mAbs 7D4 and PC61 (Cat. no. 553866), and it blocks binding of IL-2 to CD25. Immunohistochemical staining of CD25 positive cells. Frozen sections of mouse thymus were reacted with the anti-CD25 antibody. Immature thymocytes expressing the IL-2 receptor can be identified by the brown labeling of their cell membranes. Amplification 20x.
BD Pharmingen™ Biotin Rat Anti-Mouse CD25 - Biotin - Clone 7D4 - Isotype Rat IgM, κ - Reactivity Ms - 1 mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- IL-2-dependent BALB/c mouse helper T-cell clone HT-2
- 克隆位点
- 7D4
- 亚型
- IgM kappa
- Top Product
- Discover our top product IL2RA Primary Antibody
-
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody
IL2RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B5D12 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibodyIL2RA 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibodyIL2RA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibodyIL2RA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IL2RA-2395 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 PerCP
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-235) antibodyIL2RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 125 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
-
-
: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, (1995) (PubMed).
: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).
: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, (1990) (PubMed).
: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, (1989) (PubMed).
: "Expression of interleukin 2 receptor on murine fetal thymocytes." in: European journal of immunology, Vol. 15, Issue 5, pp. 456-60, (1985) (PubMed).
: "The murine IL 2 receptor. III. Cellular requirements for the induction of IL 2 receptor expression on T cell subpopulations." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 4, pp. 2405-13, (1985) (PubMed).
-
: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, (1995) (PubMed).
-
- 抗原
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 别名
- CD25 (IL2RA 产品)
- 别名
- CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
- 背景
- Synonyms: IL-2 Receptor α chain, p55
- 途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
-
You are here:
