FASL 抗体
(3 references)Quick Overview for FASL 抗体 (ABIN2689020)
抗原
See all FASL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary, and lung. FasL is a 40 kDa TNF family membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. NOK-2 recognizes human FasL. It recognizes both the membrane bound (FasL) and soluble (sFasL) forms. L5178Y mouse T lymphoma cells expressing recombinant human FasL were used as immunogen. FasL and sFasL migrate at reduced molecular weights of 40 and 26 kDa, respectively. However, the molecular weights observed in a particular sample may vary according to FasL and sFasL glycosylation and breakdown patterns as described in Tanaka et al. Immunoprecipitation of Fas Ligand (FasL). T lymphoma L5187Y cells were transfected with human FasL cDNA and treated or not treated with metalloprotease inhibitor KB8301. KB8301 blocks FasL cleavage resulting in high levels of cell surface FasL. FasL is detected in S-labeled cells treated with inhibitor KB8301 (lane 1), but not in untreated cells (lane 3). Isotype (negative) controls are shown in lanes 2 and 4. Purified NOK-2 (Cat. No. 556376) or mouse IgG2a (Cat. No. 553454) was used for immunoprecipitations.
BD Pharmingen™ Purified Mouse Anti-Human CD178 - Purified - Clone NOK-2 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- L51788Y Mouse T Lymphoma Cells Expressing Recombinant Human FasL
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亚型
- IgG2a
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anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 196-281) antibody
FASL 适用: 人, 小鼠, 大鼠, Pig WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-200) antibodyFASL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 102-280) antibodyFASL 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 100-278) antibodyFASL 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 103-281) antibodyFASL 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 82-145) antibodyFASL 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) antibodyFASL 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2G9-G8 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC, Func 宿主: 小鼠 Monoclonal NOK-1 unconjugated
anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibodyFASL 适用: 人 WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal NOK-1 unconjugated
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应用备注
- Flow Cytometry : Another clone, NOK-1, (556372, 556373) are recommended for flow cytometry. NOK-2 appears weaker for flow cytometry than NOK-1. Western Blot: Neither NOK-1 nor NOK-2 are suggested for western blot analysis. Another human FasL clone, G247-4 is suggested for the western blot analysis.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, (1996) (PubMed).
: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).
: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).
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: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, (1996) (PubMed).
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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别名
- CD178
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背景
- Synonyms: Fas Ligand, CD95 Ligand
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分子量
- 40, 26 kDa
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途径
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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