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CD40 Ligand 抗体

Name: CD40 Ligand 抗体
SKU: ABIN2688993
Availability: OutOfStock

This anti-CD40 Ligand antibody is a Armenian Hamster 单克隆 antibody detecting CD40 Ligand in FACS, BR 和 IHC (fro). Suitable for 小鼠. This Primary Antibody has been cited in 23+ publications.

(23 references)

产品编号 ABIN2688993

发货至: 中国

Quick Overview for CD40 Ligand 抗体 (ABIN2688993)

抗原

See all CD40 Ligand (CD40LG) 抗体

CD40 Ligand (CD40LG)

适用

小鼠

宿主

Armenian Hamster

克隆类型

单克隆

标记

This CD40 Ligand antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

MR1

Discover our top product CD40LG Primary Antibody

品牌

BD Pharmingen™

产品特性

The MR1 antibody reacts with CD154 (CD40 Ligand, gp39), an accessory molecule expressed on activated T helper (CD4+) lymphocytes. CD154 has also been detected on other types of leukocytes, including CD8+ T cells, medullary thymocytes, activated CD4+ NK-T cells, and human NK cells. CD154 plays an important role in costimulatory interactions between T and B lymphocytes and between antigen-presenting cells and lymphocytes, regulating the immune response at multiple levels. MR1 mAb inhibits in vitro activation of B lymphocytes by T helper cells by blocking interaction of gp39 with CD40. In vitro interactions of T cells and antigen-presenting cells can also be blocked by the MR1 antibody. In vivo treatment with MR1 antibody blocks the development of experimental autoimmune disease, inhibits formation of germinal centers and generation of memory B cells, reduces T-lymphocyte responses to allogeneic cells and allografts, prevents intrathymic deletion of self-reactive T lymphocytes, and disrupts antigen-specific T-cell responses. CD154 expression on activated and resting T lymphocytes. BALB/c spleen T cells, purified on a T Cell Enrichment Column (R&D Systems), were cultured for 8 hours in the presence (Panels A and C) or absence (Panel B) of plate-bound 500A2 antibody (anti-CD3e, Cat. No. 553238). They were stained with purified MR1 antibody (Panels A and B), followed by biotinylated mouse anti-hamster IgG, Cat. No. 554010, then Streptavidin-PE, Cat. No. 554061, (Panels A, B, and C). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Hamster Anti-Mouse CD154 - Purified - Clone MR1 - Isotype Armenian Hamster IgG3, κ - Reactivity Ms - 0.5 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Activated mouse Th1 clone D1.6

亚型

IgG3 kappa
anti-CD40 Ligand (CD40LG) (AA 1-261) antibody
CD40LG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2763 unconjugated

anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2761 unconjugated

anti-CD40 Ligand (CD40LG) antibody (FITC)
CD40LG 适用: 小鼠 FACS 宿主: 仓鼠 Monoclonal MR-1 FITC

anti-CD40 Ligand (CD40LG) (AA 79-129) antibody
CD40LG 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 33-62) antibody
CD40LG 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 111-261) antibody
CD40LG 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) antibody (PE)
CD40LG 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 24-31 PE

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 8H10F5 unconjugated

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 5A3A9 unconjugated

应用备注

Flow Cytometry System.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Miga, Masters, Durell, Gonzalez, Jenkins, Maliszewski, Kikutani, Wade, Noelle: "Dendritic cell longevity and T cell persistence is controlled by CD154-CD40 interactions." in: European journal of immunology, Vol. 31, Issue 3, pp. 959-65, (2001) (PubMed).

Graca, Honey, Adams, Cobbold, Waldmann: "Cutting edge: anti-CD154 therapeutic antibodies induce infectious transplantation tolerance." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 4783-6, (2000) (PubMed).

Lettesjö, Burd, Mageed: "CD4+ T lymphocytes with constitutive CD40 ligand in preautoimmune (NZB x NZW)F1 lupus-prone mice: phenotype and possible role in autoreactivity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 4095-104, (2000) (PubMed).

Tomura, Yu, Ahn, Yamashita, Yang, Ono, Hamaoka, Kawano, Taniguchi, Koezuka, Fujiwara: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, (1999) (PubMed).

Garside, Ingulli, Merica, Johnson, Noelle, Jenkins: "Visualization of specific B and T lymphocyte interactions in the lymph node." in: Science (New York, N.Y.), Vol. 281, Issue 5373, pp. 96-9, (1998) (PubMed).

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." in: Annual review of immunology, Vol. 16, pp. 111-35, (1998) (PubMed).

Kalled, Cutler, Datta, Thomas: "Anti-CD40 ligand antibody treatment of SNF1 mice with established nephritis: preservation of kidney function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 5, pp. 2158-65, (1998) (PubMed).

Carbone, Ruggiero, Terrazzano, Palomba, Manzo, Fontana, Spits, Kärre, Zappacosta: "A new mechanism of NK cell cytotoxicity activation: the CD40-CD40 ligand interaction." in: The Journal of experimental medicine, Vol. 185, Issue 12, pp. 2053-60, (1997) (PubMed).

DeKruyff, Gieni, Umetsu: "Antigen-driven but not lipopolysaccharide-driven IL-12 production in macrophages requires triggering of CD40." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 1, pp. 359-66, (1997) (PubMed).

Dunn, Luedecker, Haugen, Clegg, Farr: "Thymic overexpression of CD40 ligand disrupts normal thymic epithelial organization." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 45, Issue 1, pp. 129-41, (1997) (PubMed).

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." in: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, (1997) (PubMed).

Kelsall, Stüber, Neurath, Strober: "Interleukin-12 production by dendritic cells. The role of CD40-CD40L interactions in Th1 T-cell responses." in: Annals of the New York Academy of Sciences, Vol. 795, pp. 116-26, (1997) (PubMed).

Masten, Yates, Pollard Koga, Lipscomb: "Characterization of accessory molecules in murine lung dendritic cell function: roles for CD80, CD86, CD54, and CD40L." in: American journal of respiratory cell and molecular biology, Vol. 16, Issue 3, pp. 335-42, (1997) (PubMed).

Griggs, Agersborg, Noelle, Ledbetter, Linsley, Tung: "The relative contribution of the CD28 and gp39 costimulatory pathways in the clonal expansion and pathogenic acquisition of self-reactive T cells." in: The Journal of experimental medicine, Vol. 183, Issue 3, pp. 801-10, (1996) (PubMed).

Laman, Claassen, Noelle: "Functions of CD40 and its ligand, gp39 (CD40L)." in: Critical reviews in immunology, Vol. 16, Issue 1, pp. 59-108, (1996) (PubMed).

Larsen, Elwood, Alexander, Ritchie, Hendrix, Tucker-Burden, Cho, Aruffo, Hollenbaugh, Linsley, Winn, Pearson: "Long-term acceptance of skin and cardiac allografts after blocking CD40 and CD28 pathways." in: Nature, Vol. 381, Issue 6581, pp. 434-8, (1996) (PubMed).

Foy, Page, Waldschmidt, Schoneveld, Laman, Masters, Tygrett, Ledbetter, Aruffo, Claassen, Xu, Flavell, Oehen, Hedrick, Noelle: "An essential role for gp39, the ligand for CD40, in thymic selection." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1377-88, (1995) (PubMed).

Roy, Aruffo, Ledbetter, Linsley, Kehry, Noelle: "Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses." in: European journal of immunology, Vol. 25, Issue 2, pp. 596-603, (1995) (PubMed).

Tian, Noelle, Lawrence: "Activated T cells enhance nitric oxide production by murine splenic macrophages through gp39 and LFA-1." in: European journal of immunology, Vol. 25, Issue 1, pp. 306-9, (1995) (PubMed).

Foy, Laman, Ledbetter, Aruffo, Claassen, Noelle: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." in: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, (1994) (PubMed).

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抗原

CD40 Ligand (CD40LG)

别名

CD154

背景

Synonyms: CD40 Ligand, gp39

途径

NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints