Angiotensin I Converting Enzyme 1 抗体
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Quick Overview for Angiotensin I Converting Enzyme 1 抗体 (ABIN2688976)
抗原
See all Angiotensin I Converting Enzyme 1 (ACE) 抗体适用
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克隆类型
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应用范围
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产品特性
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The BB9 Monoclonal antibody specifically binds Angiotensin-converting enzyme (ACE) which is also known as Dipeptidyl carboxypeptidase 1 (DCP1) or CD143. CD143 is a type I transmembrane glycoprotein that functions as a zinc metallopeptidase involved with the metabolism of angiotensin, bradykinin, and other oligopeptides. It is expressed on primitive hematopoietic progenitor cells (HPC). BB9 reacts with stromal cells, a minor subset of human bone marrow cells, it does not react with peripheral blood leucocytes. BB9 recognizes a subset of CD34+CD90+ cells that demonstrate low or absent expression of CD38, low retention of Rhodamine 123 and are Ki-67-negative. CD34+BB9+ cells are able to sustain hematopoiesis in pre-CFU culture stimulated with IL-3, IL-6, G-CSF, and SCF. BB9 also reacts with a subset of CD34+ cells from mobilized peripheral blood. This antibody was generated using human bone marrow-derived stromal cells as immunogen. Flow cytometric analysis of CD143 expression on human bone marrow cells. Human bone marrow cells were stained with APC Anti-Human CD34 (hematopoietic progenitor cell marker) and Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis of CD143 expression on human bone marrow cell line. HEL 92.1.7 (Human bone marrow erythroblast, ATCC TIB-180) cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746, Dashed line histogram) or Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) (Solid line historgram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells.
BD Pharmingen™ Purified Mouse Anti-Human Angiotensin Converting Enzyme (CD143) - Purified - Clone BB9 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human bone marrow-derived stromal cells
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亚型
- IgG1 kappa
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anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 1160-1306) antibody
ACE 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 814-1071) antibodyACE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 651-864) antibodyACE 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IHC (fro), IF 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 801-900) antibodyACE 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, FACS, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 702-715), (Middle Region) antibodyACE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 30-270) antibodyACE 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 592-701) antibodyACE 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4B10 unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 592-701) antibodyACE 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6A4 unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) antibodyACE 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal 9B9 unconjugated
anti-Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 30-182) antibodyACE 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3H7E4 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C.
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: "Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human embryo." in: Blood, Vol. 119, Issue 16, pp. 3712-23, (2012) (PubMed).
: "Expression of angiotensin-converting enzyme (CD143) identifies and regulates primitive hemangioblasts derived from human pluripotent stem cells." in: Blood, Vol. 112, Issue 9, pp. 3601-14, (2008) (PubMed).
: "Angiotensin-converting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult hematopoietic tissues." in: Blood, Vol. 111, Issue 8, pp. 4055-63, (2008) (PubMed).
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: "Angiotensin-converting enzyme (CD143) specifies emerging lympho-hematopoietic progenitors in the human embryo." in: Blood, Vol. 119, Issue 16, pp. 3712-23, (2012) (PubMed).
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- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
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别名
- CD143
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背景
- Synonyms: ACE1, DCP1, Kininase II
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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