BMI1 抗体
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北京 101111
Quick Overview for BMI1 抗体 (ABIN2668619)
抗原
See all BMI1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- BMI-1 antibody (mAb) (Clone AF27)
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纯化方法
- Protein G Chromatography
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免疫原
- This BMI-1 antibody was raised against a recombinant protein corresponding to full-length mouse BMI-1.
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亚型
- IgG2a
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anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) antibody
BMI1 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB22858 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) antibodyBMI1 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 250-300) antibodyBMI1 适用: 人, 小鼠 WB, IHC, ICC, IP, FACS 宿主: 兔 Monoclonal BLR119H unconjugated Recombinant Antibody
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 142-326) antibodyBMI1 适用: 人, 小鼠 WB, IHC, IF 宿主: 小鼠 Monoclonal BMI1-2823 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 120-326) antibodyBMI1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 142-326) antibodyBMI1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal BMI1-2689 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 1-326) antibodyBMI1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4E10-1C5 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 142-326) antibodyBMI1 适用: 人 IHC 宿主: 小鼠 Monoclonal BMI1-2690 unconjugated
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) antibodyKD Validated BMI1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB685 unconjugated Recombinant Antibody
anti-BMI1 Polycomb Ring Finger Oncogene (BMI1) (AA 257-286), (C-Term) antibodyBMI1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB02926 unconjugated
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应用备注
- ChIP-Seq: 4 μg per ChIP WB: 0.5 - 2 μg/mL For optimal results, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Senescence associated MCP-1 secretion is dependent on a decline in BMI1 in human mesenchymal stromal cells." in: Antioxidants & redox signaling, (2015) (PubMed).
: "Notch3 marks clonogenic mammary luminal progenitor cells in vivo." in: The Journal of cell biology, Vol. 203, Issue 1, pp. 47-56, (2013) (PubMed).
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- BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))
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别名
- BMI-1
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背景
- BMI-1 is a member of the Polycomb PRC1 complex that is recruited to transcriptionally repressed genes subsequent to histone H3 lysine 27 methylation in order to maintain repression. In the PRC1 complex, BMI-1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2, resulting in the ubiquitylation of histone H2A at lysine 119. BMI-1 acts as an oncogene and cooperates with c-myc in the initiation of lymphoma. High levels of expression of BMI-1 are observed in metastatic melanoma, and BMI-1 has been implicated in several other cancers. BMI-1 is required for hematopoietic stem cell self renewal.
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分子量
- 40 kDa
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基因ID
- 6605
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NCBI登录号
- NP_031578
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途径
- Cell Division Cycle, Autophagy
抗原
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