Rpb1 CTD 抗体 (pThr4)
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北京 101111
Quick Overview for Rpb1 CTD 抗体 (pThr4) (ABIN2668543)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pThr4
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原理
- RNA pol II CTD phospho Thr4 antibody (mAb) (Clone 6D7)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.
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亚型
- IgG2b
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anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer7) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated
anti-RNA Polymerase II CTD (pThr4) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, Saccharomyces cerevisiae WB, IF, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E10 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E8 unconjugated
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应用备注
- WB: 0.5 - 2 μg/mL DB: 1 μg/mL
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).
: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).
: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).
: "CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II." in: Science (New York, N.Y.), Vol. 336, Issue 6089, pp. 1723-5, (2012) (PubMed).
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- Rpb1 CTD (RNA Polymerase II CTD)
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别名
- RNA Pol II CTD
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背景
- RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.
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分子量
- 240 kDa
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基因ID
- 5430
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NCBI登录号
- NP_000928
抗原
-
