Rpb1 CTD 抗体 (pSer5)
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北京 101111
Quick Overview for Rpb1 CTD 抗体 (pSer5) (ABIN2668520)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer5
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原理
- RNA pol II CTD phospho Ser5 antibody (pAb)
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纯化方法
- Protein A Chromatography
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免疫原
- This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.
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亚型
- IgG
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anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer7) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated
anti-RNA Polymerase II CTD (pThr4) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, Saccharomyces cerevisiae WB, IF, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E10 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E8 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP: 4 - 10 μg per ChIP ChIP-Seq: 4 µL each WB: 0.5 - 2 μg/mL dilution For RNA pol II CTD phospho Ser5, we also offer AbFlex RNA pol II CTD phospho Ser5 Recombinant Antibody (rAb). For details, see Catalog No. 91119.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast." in: BMC genomics, Vol. 18, Issue 1, pp. 107, (2017) (PubMed).
: "IUGR increases chromatin-remodeling factor Brg1 expression and binding to GR exon 1.7 promoter in newborn male rat hippocampus." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 309, Issue 2, pp. R119-27, (2015) (PubMed).
: "The head module of Mediator directs activation of preloaded RNAPII in vivo." in: Nucleic acids research, Vol. 41, Issue 22, pp. 10124-34, (2013) (PubMed).
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- Rpb1 CTD (RNA Polymerase II CTD)
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别名
- RNA Pol II CTD
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背景
- RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.
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分子量
- 210 kDa
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基因ID
- 5430
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NCBI登录号
- NP_000928
抗原
-
