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Rpb1 CTD 抗体 (pSer5)

Name: Rpb1 CTD 抗体 (pSer5)
SKU: ABIN4889651
Availability: OutOfStock

This 兔多克隆 antibody specifically detects Rpb1 CTD in WB, IF, ICC 和 ChIP. It exhibits reactivity toward 人和小鼠. It has been mentioned in 5+ publications

RNA pol II CTD phospho Ser5 antibody tested by Western blot. (Rpb1 CTD 抗体 (pSer5))

RNA pol II CTD phospho Ser5 antibody tested by immunofluorescence. (Rpb1 CTD 抗体 (pSer5))

RNA pol II CTD phospho Ser5 pAb tested by dot blot analysis. (Rpb1 CTD 抗体 (pSer5))

ChIP

4 images

(5 references)

产品编号 ABIN4889651

发货至: 中国

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中国
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Quick Overview for Rpb1 CTD 抗体 (pSer5) (ABIN4889651)

抗原

Rpb1 CTD (RNA Polymerase II CTD)

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This Rpb1 CTD antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

抗原表位
8

5

3

2

2

2

1

1

1

1

1
pSer5

原理

RNA pol II CTD phospho Ser5 antibody (pAb)

纯化方法

Unpurified

免疫原

This RNA pol II CTD phospho Ser5 antibody was raised against synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at serine 5.
anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II CTD (pSer7) antibody
适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated

anti-RNA Polymerase II CTD (pSer2) antibody
适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated

anti-RNA Polymerase II CTD (pThr4) antibody
适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, Saccharomyces cerevisiae WB, IF, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 8A7 unconjugated

anti-RNA Polymerase II CTD (pSer2) antibody
适用: 人, 小鼠 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E10 unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E8 unconjugated

anti-RNA Polymerase II CTD (pSer2) antibody
适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 10 µL per ChIP ICC/IF: 1:250 - 1:500 dilution WB: 1:500 - 1:2,000 dilution For RNA pol II CTD phospho Ser5, we also offer AbFlex RNA pol II CTD phospho Ser5 Recombinant Antibody (rAb). For details, see Catalog No. 91119.

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Schubert, Weisshart: "Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei." in: Journal of experimental botany, Vol. 66, Issue 6, pp. 1687-98, (2015) (PubMed).

Albini, Coutinho, Malecova, Giordani, Savchenko, Forcales, Puri: "Epigenetic reprogramming of human embryonic stem cells into skeletal muscle cells and generation of contractile myospheres." in: Cell reports, Vol. 3, Issue 3, pp. 661-70, (2013) (PubMed).

Sanei, Pickering, Kumke, Nasuda, Houben: "Loss of centromeric histone H3 (CENH3) from centromeres precedes uniparental chromosome elimination in interspecific barley hybrids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 33, pp. E498-505, (2011) (PubMed).

Nelissen, De Groeve, Fleury, Neyt, Bruno, Bitonti, Vandenbussche, Van der Straeten, Yamaguchi, Tsukaya, Witters, De Jaeger, Houben, Van Lijsebettens: "Plant Elongator regulates auxin-related genes during RNA polymerase II transcription elongation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 4, pp. 1678-83, (2010) (PubMed).

Blythe, Cha, Tadjuidje, Heasman, Klein: "beta-Catenin primes organizer gene expression by recruiting a histone H3 arginine 8 methyltransferase, Prmt2." in: Developmental cell, Vol. 19, Issue 2, pp. 220-31, (2010) (PubMed).
抗原

Rpb1 CTD (RNA Polymerase II CTD)

别名

RNA Pol II CTD

背景

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at Serine 5 and Serine 2. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

分子量

210 kDa

基因ID

5430

NCBI登录号

NP_000928