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Rpb1 CTD 抗体 (pSer5)

Name: Rpb1 CTD 抗体 (pSer5)
SKU: ABIN2668541
Availability: OutOfStock

Cited in 5+ publications. The 大鼠单克隆 anti-Rpb1 CTD antibody (Clone 3E8) (ABIN2668541) specifically detects Rpb1 CTD in WB, IF, ICC, ChIP, ChIP-seq 和 IP. The antibody is reactive with 人 samples.

ChIP-seq

ChIP

2 images

(5 references)

产品编号 ABIN2668541

发货至: 中国

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Quick Overview for Rpb1 CTD 抗体 (pSer5) (ABIN2668541)

抗原

Rpb1 CTD (RNA Polymerase II CTD)

适用

人

宿主

大鼠

克隆类型

单克隆

标记

This Rpb1 CTD antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Immunoprecipitation (IP)

克隆位点

3E8

抗原表位
8

5

3

2

2

2

1

1

1

1
pSer5

原理

RNA pol II CTD phospho Ser5 antibody (mAb) (Clone 3E8)

纯化方法

Protein G Chromatography

免疫原

This RNA pol II CTD phospho Ser5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

亚型

IgG2a
anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II CTD (pSer7) antibody
适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated

anti-RNA Polymerase II CTD (pSer2) antibody
适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated

anti-RNA Polymerase II CTD (pThr4) antibody
适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, Saccharomyces cerevisiae WB, IF, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 8A7 unconjugated

anti-RNA Polymerase II CTD (pSer2) antibody
适用: 人, 小鼠 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E10 unconjugated

anti-RNA Polymerase II CTD (pSer2) antibody
适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 5-10 μg per ChIP ChIP-Seq: 5-10 μg each IF: 1:500 dilution WB*: 0.5 - 2 μg/mL dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. In addition, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background.

限制

仅限研究用
浓度

1 μg/μL

缓冲液

Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Harlen, Trotta, Smith, Mosaheb, Fuchs, Churchman: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).

Ramos, Wijetunga, McLellan, Suzuki, Greally: "DNA demethylation by 5-aza-2'-deoxycytidine is imprinted, targeted to euchromatin, and has limited transcriptional consequences." in: Epigenetics & chromatin, Vol. 8, pp. 11, (2015) (PubMed).

Eifler, Shao, Bartholomeeusen, Fujinaga, Jäger, Johnson, Luo, Krogan, Peterlin: "Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 468-78, (2014) (PubMed).

Hintermair, Heidemann, Koch, Descostes, Gut, Gut, Fenouil, Ferrier, Flatley, Kremmer, Chapman, Andrau, Eick: "Threonine-4 of mammalian RNA polymerase II CTD is targeted by Polo-like kinase 3 and required for transcriptional elongation." in: The EMBO journal, Vol. 31, Issue 12, pp. 2784-97, (2012) (PubMed).

Chapman, Heidemann, Albert, Mailhammer, Flatley, Meisterernst, Kremmer, Eick: "Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7." in: Science (New York, N.Y.), Vol. 318, Issue 5857, pp. 1780-2, (2007) (PubMed).
抗原

Rpb1 CTD (RNA Polymerase II CTD)

别名

RNA Pol II CTD

背景

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.

分子量

240 kDa

基因ID

5430

NCBI登录号

NP_000928