Rpb1 CTD 抗体 (pTyr1)
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北京 101111
Quick Overview for Rpb1 CTD 抗体 (pTyr1) (ABIN2668519)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pTyr1
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原理
- RNA pol II CTD phospho Tyr1 antibody (mAb) (Clone 3D12)
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纯化方法
- Protein G Chromatography
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免疫原
- This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1.
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亚型
- IgG1
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anti-RNA Polymerase II CTD (pSer5) antibody
适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, 小鼠 WB, ChIP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer7) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3D4A12 unconjugated
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 1H4B6 unconjugated
anti-RNA Polymerase II CTD (pThr4) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II CTD (pSer5) antibody适用: 人, Saccharomyces cerevisiae WB, IF, ICC, ELISA, FACS 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-RNA Polymerase II CTD (pTyr1) antibody (Sortase,His tag,AVI tag)适用: 人 WB, IF, ChIP-seq, ELISA 宿主: 小鼠 Monoclonal Sortase,His tag,AVI tag Recombinant Antibody
anti-RNA Polymerase II CTD (pSer2) antibody适用: 人, 小鼠 WB, IF, ICC, ChIP, ChIP-seq, IP 宿主: 大鼠 Monoclonal 3E10 unconjugated
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应用备注
- WB*: 1 - 2 μg/mL *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. In addition, we recommend the addition of 0.05 % Tween 20 to all blocking solutions to reduce background. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91219.
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Comprehensive RNA Polymerase II Interactomes Reveal Distinct and Varied Roles for Each Phospho-CTD Residue." in: Cell reports, Vol. 15, Issue 10, pp. 2147-2158, (2016) (PubMed).
: "CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 396-403, (2015) (PubMed).
: "Herpes Simplex Virus 1 (HSV-1) ICP22 protein directly interacts with cyclin-dependent kinase (CDK)9 to inhibit RNA polymerase II transcription elongation." in: PLoS ONE, Vol. 9, Issue 9, pp. e107654, (2014) (PubMed).
: "CTD tyrosine phosphorylation impairs termination factor recruitment to RNA polymerase II." in: Science (New York, N.Y.), Vol. 336, Issue 6089, pp. 1723-5, (2012) (PubMed).
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- Rpb1 CTD (RNA Polymerase II CTD)
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别名
- RNA Pol II CTD
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背景
- RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at sites within the heptapeptide repeat, at serine 2, serine 5, serine 7 and tyrosine 1. RNA pol II Tyrosine 1 phosphorylation is found during elongation and appears to activate transcription by suppressing RNA pol II termination.
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分子量
- 210 kDa
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基因ID
- 5430
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NCBI登录号
- NP_000928
抗原
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