Histone 3 抗体 (H3K18ac)
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北京 101111
Quick Overview for Histone 3 抗体 (H3K18ac) (ABIN2668428)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- H3K18ac
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原理
- Histone H3K18ac antibody (pAb)
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纯化方法
- Protein A Chromatography
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免疫原
- This Histone H3 acetyl Lys18 antibody was raised against a peptide including acetyl-lysine 18 of histone H3.
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亚型
- IgG
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应用备注
- ChIP: 10 μg per ChIP ChIP-Seq: 4 μg each ICC/IF: 1 μg/mL dilution WB: 0.5 - 2 μg/mL dilution DB: 0.1 - 0.5 μg/mL dilution
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS ( pH 7.5) with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).
: "HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT)." in: PLoS pathogens, Vol. 12, Issue 1, pp. e1005372, (2016) (PubMed).
: "The microtubule-associated tau protein has intrinsic acetyltransferase activity." in: Nature structural & molecular biology, Vol. 20, Issue 6, pp. 756-62, (2013) (PubMed).
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.
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分子量
- 17 kDa
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基因ID
- 3020
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NCBI登录号
- NP_003522
抗原
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