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Histone 3 抗体 (H3K4me2)

Name: Histone 3 抗体 (H3K4me2)
SKU: ABIN2668425
Availability: OutOfStock

This 兔多克隆 antibody specifically detects Histone 3 in WB, ChIP, DB, ChIP-seq 和 CUT&Tag. It exhibits reactivity toward 人和小鼠. It has been mentioned in 15+ publications

Histone H3 dimethyl Lys4 antibody tested by Western blot. (Histone 3 抗体 (H3K4me2))

Histone H3 dimethyl Lys4 pAb tested by dot blot analysis. (Histone 3 抗体 (H3K4me2))

ChIP-seq

ChIP

Histone H3 dimethyl Lys4 antibody specificity tested by peptide array analysis. (Histone 3 抗体 (H3K4me2))

PAr

5 images

(15 references)

产品编号 ABIN2668425

发货至: 中国

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Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Histone 3 抗体 (H3K4me2) (ABIN2668425)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

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人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Discover our top product H3 Primary Antibody

抗原表位
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H3K4me2

原理

Histone H3K4me2 antibody (pAb)

纯化方法

Unpurified

免疫原

This Histone H3 dimethyl Lys4 antibody was raised against a peptide including dimethyl-lysine 4 of histone H3.
anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 5 - 10 µL per ChIP ChIP-Seq: 3 µL each WB*: 1:1,000 - 1:5,000 dilution CUT&Tag: 1 µL per 50 µL reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H3K4me2, we also offer AbFlex Histone H3K4me2 Recombinant Antibody (rAb). For details, see Catalog No. 91321.

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Dheekollu, Wiedmer, Sentana-Lledo, Cassel, Messick, Lieberman: "HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus." in: Journal of virology, Vol. 90, Issue 11, pp. 5353-67, (2016) (PubMed).

Zhan, Kost-Alimova, Shi, Leo, Bardenhagen, Shepard, Appikonda, Vangamudi, Zhao, Tieu, Jiang, Heffernan, Marszalek, Toniatti, Draetta, Tyler, Barton, Jones, Palmer, Geck Do, Andersen: "Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery." in: Epigenetics & chromatin, Vol. 8, pp. 37, (2015) (PubMed).

Mohan, Dialynas, Weake, Liu, Martin-Brown, Florens, Washburn, Workman, Abmayr: "Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration." in: Genes & development, Vol. 28, Issue 3, pp. 259-72, (2014) (PubMed).

Walter, Matter, Fahrenkrog: "Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae." in: PLoS genetics, Vol. 10, Issue 1, pp. e1004095, (2014) (PubMed).

Konovalov, Garcia-Bassets: "Analysis of the levels of lysine-specific demethylase 1 (LSD1) mRNA in human ovarian tumors and the effects of chemical LSD1 inhibitors in ovarian cancer cell lines." in: Journal of ovarian research, Vol. 6, Issue 1, pp. 75, (2014) (PubMed).

Rafehi, Balcerczyk, Lunke, Kaspi, Ziemann, Kn, Okabe, Khurana, Ooi, Khan, Du, Chang, Haviv, Keating, Karagiannis, El-Osta: "Vascular histone deacetylation by pharmacological HDAC inhibition." in: Genome research, Vol. 24, Issue 8, pp. 1271-84, (2014) (PubMed).

Kallestad, Woods, Christensen, Gefroh, Balakrishnan, Milavetz: "Transcription and replication result in distinct epigenetic marks following repression of early gene expression." in: Frontiers in genetics, Vol. 4, pp. 140, (2013) (PubMed).

Cannuyer, Loriot, Parvizi, De Smet: "Epigenetic hierarchy within the MAGEA1 cancer-germline gene: promoter DNA methylation dictates local histone modifications." in: PLoS ONE, Vol. 8, Issue 3, pp. e58743, (2013) (PubMed).

Cie?lik, Hoang, Baranova, Chodaparambil, Kumar, Allison, Xu, Wamsley, Gray, Jones, Mayo, Bekiranov: "Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 28, (2013) (PubMed).

Jamieson, Rountree, Lewis, Stajich, Selker: "Regional control of histone H3 lysine 27 methylation in Neurospora." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 15, pp. 6027-32, (2013) (PubMed).

Subrahmanyam, Du, Ivanova, Chakraborty, Ji, Zhang, Alt, Schatz, Sen: "Localized epigenetic changes induced by DH recombination restricts recombinase to DJH junctions." in: Nature immunology, Vol. 13, Issue 12, pp. 1205-12, (2012) (PubMed).

Milavetz, Kallestad, Gefroh, Adams, Woods, Balakrishnan: "Virion-mediated transfer of SV40 epigenetic information." in: Epigenetics, Vol. 7, Issue 6, pp. 528-34, (2012) (PubMed).

Liang, He, Zhang: "Kdm2b promotes induced pluripotent stem cell generation by facilitating gene activation early in reprogramming." in: Nature cell biology, Vol. 14, Issue 5, pp. 457-66, (2012) (PubMed).

Chandrasekharan, Huang, Chen, Sun: "Histone H2B C-terminal helix mediates trans-histone H3K4 methylation independent of H2B ubiquitination." in: Molecular and cellular biology, Vol. 30, Issue 13, pp. 3216-32, (2010) (PubMed).

Honda, Selker: "Direct interaction between DNA methyltransferase DIM-2 and HP1 is required for DNA methylation in Neurospora crassa." in: Molecular and cellular biology, Vol. 28, Issue 19, pp. 6044-55, (2008) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.

分子量

17 kDa

基因ID

3020

NCBI登录号

NP_003522