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Histone 3 抗体 (meLys27)

Name: Histone 3 抗体 (meLys27)
SKU: ABIN2668399
Availability: OutOfStock

There are 3+ publications for this product available. The 小鼠单克隆 anti-Histone 3 antibody is suitable to detect Histone 3 in samples from 人. It has been validated for WB, IF, ChIP, ICC, DB, ChIP-seq 和 CUT&Tag.

Histone H3K27me1 antibody (mAb) tested by Western blot. (Histone 3 抗体 (meLys27))

ChIP

Histone H3K27me1 antibody (mAb) tested by dot blot analysis. (Histone 3 抗体 (meLys27))

ChIP-seq

4 images

(3 references)

产品编号 ABIN2668399

发货至: 中国

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Quick Overview for Histone 3 抗体 (meLys27) (ABIN2668399)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

1796
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人

宿主

小鼠

克隆类型

单克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

克隆位点

MABI 0321

Discover our top product H3 Primary Antibody

抗原表位
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meLys27

原理

Histone H3K27me1 antibody (mAb) (Clone MABI 0321)

纯化方法

Protein G Chromatography

免疫原

This Histone H3 monomethyl Lys27 antibody was raised against a peptide containing monomethyl Lys27 of human Histone H3.

亚型

IgG2a
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 2 - 5 μg per ChIP ChIP-Seq: 2 - 5 μg each WB: 0.5 - 2 μg/mL dilution CUT&Tag: 1-2 μg per 50 µL reaction ChIP-Seq validation was performed by Active Motif's Epigenetics Services, the complete data set is available in the UCSC Genome Browser by clicking here.

限制

仅限研究用
浓度

0.51 μg/μL

缓冲液

PBS pH 7.5 containing 30 % glycerol. 0.3M NaCl, and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Jacob, Bergamin, Donoghue, Mongeon, LeBlanc, Voigt, Underwood, Brunzelle, Michaels, Reinberg, Couture, Martienssen: "Selective methylation of histone H3 variant H3.1 regulates heterochromatin replication." in: Science (New York, N.Y.), Vol. 343, Issue 6176, pp. 1249-53, (2014) (PubMed).

Ntziachristos, Tsirigos, Welstead, Trimarchi, Bakogianni, Xu, Loizou, Holmfeldt, Strikoudis, King, Mullenders, Becksfort, Nedjic, Paietta, Tallman, Rowe, Tonon, Satoh, Kruidenier, Prinjha, Akira et al.: "Contrasting roles of histone 3 lysine 27 demethylases in acute lymphoblastic leukaemia. ..." in: Nature, Vol. 514, Issue 7523, pp. 513-7, (2014) (PubMed).

Kimura, Hayashi-Takanaka, Goto, Takizawa, Nozaki: "The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies." in: Cell structure and function, Vol. 33, Issue 1, pp. 61-73, (2008) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

分子量

17 kDa

基因ID

3020

NCBI登录号

NP_003522