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Histone 3 抗体 (H3K9ac)

Name: Histone 3 抗体 (H3K9ac)
SKU: ABIN2668378
Availability: OutOfStock

There are 3+ publications for this product available. The 小鼠单克隆 anti-Histone 3 antibody is suitable to detect Histone 3 in samples from 人和小鼠. It has been validated for WB, IF, ChIP, ICC, DB 和 ChIP-seq.

Histone H3 acetyl Lys9 antibody (mAb) tested by Western blot. (Histone 3 抗体 (H3K9ac))

ChIP

Histone H3 acetyl Lys9 antibody tested by immunofluorescence. (Histone 3 抗体 (H3K9ac))

ChIP-seq

Histone H3 acetyl Lys9 mAb tested by dot blot analysis. (Histone 3 抗体 (H3K9ac))

5 images

(3 references)

产品编号 ABIN2668378

发货至: 中国

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中国
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Quick Overview for Histone 3 抗体 (H3K9ac) (ABIN2668378)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

1796
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人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

克隆位点

1B10

Discover our top product H3 Primary Antibody

抗原表位
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H3K9ac

原理

Histone H3K9ac antibody (mAb) (Clone 1B10)

纯化方法

Protein A Chromatography

免疫原

The Histone H3 acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human Histone H3.

亚型

IgG3
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 2 - 5 μg per ChIP ChIP-Seq: 5 μg each WB: 0.2 - 2 μg/mL dilution DB: 0.2 - 2 μg/mL dilution CUT&Tag: 1 μg per 50 µL reaction NGS-QC certification: this antibody has been processed by the NGS-QC generator. For additional details, click here. For Histone H3K9ac, we also offer AbFlex Histone H3K9ac Recombinant Antibody (rAb). For details, see Catalog No. 91103.

限制

仅限研究用
浓度

1 μg/μL

缓冲液

Purified IgG in 63 mM Trisodium Citrate, 70 mM Sodium Phosphate Tribasic, 301 mM Sodium Chloride, 30 % Glycerol, and 0.035 % Sodium Azide. Sodium Azide is highly toxic. *Due to the high levels of NA+ in the antibody storage buffer, precipitation might be observed. Incubate the antibody tube at 37 °C and mix prior to use.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Tran, Nguyen, Takabe, Yamagata, Miyake: "Pre-treatment with amitriptyline causes epigenetic up-regulation of neuroprotection-associated genes and has anti-apoptotic effects in mouse neuronal cells." in: Neurotoxicology and teratology, Vol. 62, pp. 1-12, (2017) (PubMed).

Mitsuda, Shimizu: "Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161288, (2016) (PubMed).

Sanders, Hagood, Liu, Zhang, Ambalavanan, Thannickal: "Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice." in: The European respiratory journal, Vol. 43, Issue 5, pp. 1448-58, (2014) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of Lys9 is associated with trascriptional activation of genes.

分子量

17

基因ID

8352

NCBI登录号

NP_003522