Cyclin E1 抗体
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Quick Overview for Cyclin E1 抗体 (ABIN265379)
抗原
See all Cyclin E1 (CCNE1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Cyclin E1 protein (region surrounding Ile119).
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Cyclin E1 (CCNE1) (AA 10-176) antibody
CCNE1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CCNE1-2460 unconjugated
anti-Cyclin E1 (CCNE1) (AA 307-410) antibodyCCNE1 适用: 人, 小鼠 ELISA, FACS 宿主: 小鼠 Monoclonal 5F8C5 unconjugated
anti-Cyclin E1 (CCNE1) (AA 1-100) antibodyCCNE1 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1C8D4 unconjugated
anti-Cyclin E1 (CCNE1) (AA 375-411) antibodyCCNE1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E1 (CCNE1) (AA 1-410) antibodyCCNE1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E1 (CCNE1) (Thr395) antibodyCCNE1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E1 (CCNE1) antibodyCCNE1 适用: 人 IHC, ELISA, IF 宿主: 兔 Monoclonal 3A5 unconjugated Recombinant Antibody
anti-Cyclin E1 (CCNE1) (C-Term) antibodyCCNE1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E1 (CCNE1) (C-Term) antibodyCCNE1 适用: 人, 小鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin E1 (CCNE1) antibodyKD Validated CCNE1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB400 unconjugated Recombinant Antibody
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Cyclin E1 (CCNE1)
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别名
- Cyclin E1
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背景
- Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primaril during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.Synonyms: CCNE, CCNE1, Cyclin-E1
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分子量
- approx. 49 kDa
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基因ID
- 898
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NCBI登录号
- NP_001229
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UniProt
- P24864
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途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
抗原
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