Cullin 1 抗体
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- 抗原 See all Cullin 1 (CUL1) 抗体
- Cullin 1 (CUL1)
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Cullin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of Cullin-1 protein (region surrounding Tyr761).
- 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- 纯度
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product CUL1 Primary Antibody
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anti-Cullin 1 (CUL1) (AA 500-776) antibody
CUL1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-250) antibodyCUL1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (AA 1-776) antibodyCUL1 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) antibodyCUL1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 适用: 人 WB, ELISA, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Cullin 1 (CUL1) (C-Term) antibodyCUL1 适用: 人, 小鼠, 大鼠, 小鸡, Cow, 犬, Pig, 兔 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Cullin 1 (CUL1)
- 别名
- Cullin 1 (CUL1 产品)
- 别名
- LOC100282292 antibody, Cullin-1 antibody, Cul1 antibody, zgc:55747 antibody, zgc:77500 antibody, wu:fc13a10 antibody, cullin-1 antibody, ATCUL1 antibody, AUXIN RESISTANT 6 antibody, AXR6 antibody, T10P11.26 antibody, T10P11_26 antibody, cullin 1 antibody, cul1 antibody, wu:fi43h07 antibody, wu:fj43a08 antibody, zgc:111899 antibody, zgc:55483 antibody, Cul-1 antibody, cullin 1 antibody, cullin-1 antibody, Cullin 1 antibody, Cullin-1 antibody, cullin 1a antibody, cullin 1 S homeolog antibody, cullin 1b antibody, CUL1 antibody, LOC100282292 antibody, cul1 antibody, ATEG_00491 antibody, EDI_072700 antibody, EDI_264400 antibody, SJAG_02591 antibody, BDBG_03785 antibody, Cul1 antibody, cul1a antibody, cul1.S antibody, cul-1 antibody, cul1b antibody
- 背景
- CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.Synonyms: CUL-1, CUL1, Cullin-1
- 分子量
- approx. 90 kDa
- 基因ID
- 8454
- NCBI登录号
- NP_003583
- UniProt
- Q13616
- 途径
- Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size
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