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Actin 抗体

The 兔 多克隆 anti-Actin antibody (ABIN265299) specifically detects Actin in WB 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN265299
发货至: 中国
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Quick Overview for Actin 抗体 (ABIN265299)

抗原

See all Actin (ACTA1) 抗体
Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

  • 73
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多克隆

标记

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This Actin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of Actin-alpha-1 protein (region surrounding Asp3).

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    Affinity Chromatography using epitope-specific immunogen
  • 应用备注

    ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

    别名

    alpha Skeletal Muscle Actin / ACTA1

    背景

    All eukaryotic cells express actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. α actin expression is limited to various types of muscle, whereas β and γ are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

    分子量

    approx. 45 kDa

    基因ID

    58

    NCBI登录号

    NP_001091

    UniProt

    P68133

    途径

    Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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