CD42a 抗体 (FITC)
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- 抗原 See all CD42a (GP9) 抗体
- CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD42a antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody recognises human Platelet GPIX.
- 产品特性
- Synonyms: Platelet glycoprotein IX, GP-IX, GP9, Glycoprotein 9
- 克隆位点
- ESS
- 亚型
- IgG1
- Top Product
- Discover our top product GP9 Primary Antibody
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anti-Glycoprotein IX (Platelet) (GP9) (AA 1-189) antibody
GP9 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glycoprotein IX (Platelet) (GP9) antibody (FITC)GP9 适用: 人, 犬 FACS 宿主: 小鼠 Monoclonal GR-P FITC
anti-Glycoprotein IX (Platelet) (GP9) (AA 17-147) antibodyGP9 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 4E2G7 unconjugated
anti-Glycoprotein IX (Platelet) (GP9) (AA 17-88) antibodyGP9 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycoprotein IX (Platelet) (GP9) antibodyGP9 适用: 人, 犬 FACS 宿主: 小鼠 Monoclonal GR-P unconjugated
anti-Glycoprotein IX (Platelet) (GP9) antibody (APC)GP9 适用: 人, 犬 FACS 宿主: 小鼠 Monoclonal GR-P APC
anti-Glycoprotein IX (Platelet) (GP9) antibody (PE)GP9 适用: 人, 犬, Mink FACS 宿主: 小鼠 Monoclonal GRP-P PE
anti-Glycoprotein IX (Platelet) (GP9) (AA 1-177) antibodyGP9 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Glycoprotein IX (Platelet) (GP9) (Internal Region) antibodyGP9 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycoprotein IX (Platelet) (GP9) antibody (PE)GP9 适用: 人, 犬 FACS 宿主: 小鼠 Monoclonal GR-P PE
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- 应用备注
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Flow Cytometry. Identification of platelets. Identification of megakaryocytes. Diagnosis of Bernard-Soulier syndrome (CD42a-). Megakaryoblastic/cytic leukemia's (CD42a+). See Protocols for more details.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- UseConsult the appropriate Negative Control factsheet to determine the amount of antibody tobe used as a control for Platelets. Collect blood aseptically by venipuncture into an ACD or EDTA blood collection tube. Important: Within 5 minutes of blood collection, fix blood sample by placing 100 µl ofblood in test tube containing 1 ml of cold (4°C) 1X PBS with 1% paraformaldehyde. Mix by vortexing. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20°C. Aspirate the supernatant, leave pellet. Prior to staining, wash the fixed blood pellet 2X with 1 ml 1X PBS + 0. 1% Azide at RT. Centrifuge the fixed blood at 1200 x g for 5 minutes at room temperature (RT) 20°C. Aspirate the supernatant, leave pellet. Resuspend the pellet in 1 ml of 1X PBS at RT. To a clean labeled test tube add 10 µl of MAb. Carefully add 50 ul of the fixed blood suspension to the bottom of the test tube. Vortex and incubate at room temperature for at least 15 minutes and analyzed within 3hours. Please note: Using this procedure will yield twenty samples for platelet analysis. Select logarithmic (Log) amplification for both Forward (FSC) and Side (SSC) scatters, whilecollecting data for platelets. See instrument manufacturer's instructions for Immunofluorescence analysis with a flowcytometer or microscope.
- 限制
- 仅限研究用
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- 缓冲液
- PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch. - 有效期
- 12 months
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- 抗原
- CD42a (GP9) (Glycoprotein IX (Platelet) (GP9))
- 别名
- CD42a / GPIX (GP9 产品)
- 背景
- Single-chain membrane glycoprotein that forms a non-covalent complex with GPIb. (MW 23 kDa) Reactivity with resting and activated platelets, weakly on monocytes, megakaryocytes and attachment site for the platelet plasma membrane to the submembrane cytoskeleton. GPIb/IX complex, functions as the receptor for ristocetin-induced binding of von Willebrand factor and as the von Willebrand factor-depend adhesion receptor.Synonyms: GP-IX, GP9, Glycoprotein 9, Platelet glycoprotein IX
- 基因ID
- 2815
- UniProt
- P14770
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