GAPDH 抗体
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北京 101111
Quick Overview for GAPDH 抗体 (ABIN263739)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with GAPDH. Does not react with GAPDH from Dog, Cat, Mouse and Fish.
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产品特性
- Synonyms: GAPD, CDABP0047, Loading control, Glyceraldehyde-3-Phosphate Dehydrogenase
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纯化方法
- Protein A Chromatography
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免疫原
- Human or rabbit GAPDH. Hybridization of Sp2/0 myeloma cells with spleen cells fromBALB/c mice.
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亚型
- IgG1
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应用备注
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ELISA. Western Blot. Immunocytochemistry. Recommended pairs for Sandwich Immunoassay: Capture: ABIN263741Detection: ABIN263739
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 4.5 mg/mL (Sigma protein assay kit)
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缓冲液
- PBS, pH 7.4, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Store the antibody undiluted at 2-8 °C.
Shelf life: One year from despatch. -
有效期
- 12 months
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- GAPDH
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背景
- Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36 kDa subunits.Synonyms: CDABP0047, GAPD, Glyceraldehyde-3-Phosphate Dehydrogenase
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基因ID
- 2597
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UniProt
- P04406
抗原
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