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XBP1 抗体 (Internal Region)

This anti-XBP1 antibody is a 山羊 多克隆 antibody detecting XBP1 in WB, ELISA, IHC 和 IF. Suitable for 人.
产品编号 ABIN263217
发货至: 中国

Quick Overview for XBP1 抗体 (Internal Region) (ABIN263217)

抗原

See all XBP1 抗体
XBP1 (X-Box Binding Protein 1 (XBP1))

适用

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宿主

  • 70
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山羊

克隆类型

  • 68
  • 16
多克隆

标记

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This XBP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 16
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    Internal Region

    原理

    XBP1 / TREB5

    序列

    NHSWEDTFAN ELFPQ

    特异性

    This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only, The epitope for EB08557 is aa358-372.

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-NHSWEDTFANELFPQ, from the internal region of the protein sequence according to NP_001073007.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 38 kDa band observed in Nuclear lysates of cell line HeLa (calculated MW of 40.1 kDa according to NP_001073007.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour.at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:4000.

    说明

    Immunofluorescence: Expression of the protein seen in the endoplasmic reticulum, cytoplasm and nucleus of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    XBP1 (X-Box Binding Protein 1 (XBP1))

    别名

    XBP1

    背景

    XBP1, TREB5, X-box binding protein 1 , XBP2, OTTHUMP00000028682, X-box-binding protein-1

    基因ID

    7494, 22433

    NCBI登录号

    NP_001073007

    途径

    ER-Nucleus Signaling, Unfolded Protein Response
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