Fibrinogen 抗体
Quick Overview for Fibrinogen 抗体 (ABIN2620902)
抗原
See all Fibrinogen 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Mouse fibrinogen.
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纯化方法
- Purified IgG
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免疫原
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Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.
Type of Immunogen: Purified protein
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anti-Fibrinogen antibody
适用: 人, 大鼠 ELISA, IF (p), IHC (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen antibody适用: 人 IF (p), IHC (p) 宿主: 小鼠 Monoclonal 4F7 unconjugated
anti-Fibrinogen antibody适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C7 unconjugated
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应用备注
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Approved: DB, ELISA, IF
Usage: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods. for the production of immunoconjugates with a selected marker. to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Distilled water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2. No preservative added.
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储存液
- Without preservative
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
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- Fibrinogen
抗原
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