Fibrinogen 抗体
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北京 101111
Quick Overview for Fibrinogen 抗体 (ABIN458504)
抗原
See all Fibrinogen 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Goat anti Mouse fibrinogen
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特异性
- Purified IgG fraction of polyclonal Goat antiSerum to Mouse fibrinogen
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Fibrinogen antibody
适用: 人 IF (p), IHC (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen antibody适用: 人 IF (p), IHC (p) 宿主: 小鼠 Monoclonal 4F7 unconjugated
anti-Fibrinogen antibody适用: 人 WB 宿主: 小鼠 Monoclonal C7 unconjugated
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." in: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).
: "Histidine-rich glycoprotein uptake and turnover is mediated by mononuclear phagocytes." in: PLoS ONE, Vol. 9, Issue 9, pp. e107483, (2014) (PubMed).
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- Fibrinogen
抗原
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