MAPK14 抗体 (Atto 594)
Quick Overview for MAPK14 抗体 (Atto 594) (ABIN2486134)
抗原
See all MAPK14 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ~38 kDa.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Full length recombinant protein expressed in E.coli cells
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亚型
- IgG1
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anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody
MAPK14 适用: 人, 小鼠, 大鼠, 小鸡, 山羊 WB, ELISA, IHC (p), FACS, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 299-360) antibodyMAPK14 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 8G4D11 unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 141-240) antibodyMAPK14 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), ICC, FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 19-279) antibodyMAPK14 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 260-360) antibodyMAPK14 适用: 人 WB, ELISA, IP, IHC (p), PLA 宿主: 小鼠 Monoclonal 3D5 unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 295-324), (C-Term) antibodyMAPK14 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB2723 unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) antibodyMAPK14 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (C-Term) antibodyMAPK14 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 299-360) antibodyMAPK14 适用: 人, 小鼠 WB, IHC, ELISA, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (AA 295-324) antibodyMAPK14 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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- WB (1:1000)
- IHC (1:1000)
- optimal dilutions for assays should be determined by the user.
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说明
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Detects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
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别名
- p38 MAPK
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背景
- The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
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基因ID
- 1432
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NCBI登录号
- NP_001306
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UniProt
- Q16539
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
抗原
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