GIT1 抗体 (AA 375-770) (PE)
Quick Overview for GIT1 抗体 (AA 375-770) (PE) (ABIN2483510)
抗原
See all GIT1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 375-770
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特异性
- Detects ~90 kDa. Does not cross-react with GIT2.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
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亚型
- IgG1
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anti-GIT1 (GIT1) (AA 460-640) antibody
KO Validated GIT1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 470-642) antibodyGIT1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 533-561), (C-Term), (Tyr554) antibodyGIT1 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB15351 unconjugated
anti-GIT1 (GIT1) (Internal Region) antibodyGIT1 适用: 人, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibody (APC)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 APC
anti-GIT1 (GIT1) (AA 375-770) antibody (Atto 594)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 Atto 594
anti-GIT1 (GIT1) (AA 375-770) antibody (Atto 390)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 Atto 390
anti-GIT1 (GIT1) (Internal Region) antibodyGIT1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibodyGIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 unconjugated
anti-GIT1 (GIT1) (AA 375-770) antibody (HRP)GIT1 适用: 大鼠 WB, IP, IF, ICC 宿主: 小鼠 Monoclonal S39B-8 HRP
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应用备注
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN2483510 was sufficient for detection of GIT1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- GIT1
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别名
- GIT1
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背景
- G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
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基因ID
- 83709
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NCBI登录号
- NP_114002
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UniProt
- Q9Z272
抗原
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