Notch1 抗体 (AA 20-216) (PerCP)
Quick Overview for Notch1 抗体 (AA 20-216) (PerCP) (ABIN2483203)
抗原
See all Notch1 (NOTCH1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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    - 
                                            抗原表位
- AA 20-216
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                                            特异性
- Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.
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                                            交叉反应
- 人, 小鼠, 大鼠
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                                            纯化方法
- Protein G Purified
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                                            免疫原
- Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
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                                            亚型
- IgG1
 
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                                            应用备注
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                        - WB (1:1000)
- optimal dilutions for assays should be determined by the user.
 
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                                            说明
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                        1 μg/ml of ABIN2483203 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. 
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                                            限制
- 仅限研究用
 
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                                            状态
- Liquid
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                                            浓度
- 1 mg/mL
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                                            缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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                                            储存液
- Sodium azide
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                                            注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            储存条件
- 4 °C
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                                            储存方法
- Conjugated antibodies should be stored at 4°C
 
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    - Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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                                            别名
- Notch1
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                                            背景
- Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
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                                            基因ID
- 18128
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                                            NCBI登录号
- NP_032740
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                                            UniProt
- Q01705
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                                            途径
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
 抗原
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