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Notch1 抗体 (AA 20-216) (PerCP)

This anti-Notch1 antibody is a 小鼠 单克隆 antibody detecting Notch1 in WB, IF 和 ICC. Suitable for 小鼠.
产品编号 ABIN2483203
发货至: 中国

Quick Overview for Notch1 抗体 (AA 20-216) (PerCP) (ABIN2483203)

抗原

See all Notch1 (NOTCH1) 抗体
Notch1 (NOTCH1) (Notch 1 (NOTCH1))

适用

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小鼠

宿主

  • 107
  • 41
  • 6
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  • 1
小鼠

克隆类型

  • 98
  • 58
  • 2
单克隆

标记

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This Notch1 antibody is conjugated to PerCP

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

S253-32
  • 抗原表位

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    AA 20-216

    特异性

    Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Protein G Purified

    免疫原

    Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1

    亚型

    IgG1
  • 应用备注

    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μg/ml of ABIN2483203 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    Notch1 (NOTCH1) (Notch 1 (NOTCH1))

    别名

    Notch1

    背景

    Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

    基因ID

    18128

    NCBI登录号

    NP_032740

    UniProt

    Q01705

    途径

    Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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