Notch1 抗体 (AA 20-216) (Biotin)
Our Local Distributor
北京 101111
Quick Overview for Notch1 抗体 (AA 20-216) (Biotin) (ABIN2483199)
抗原
See all Notch1 (NOTCH1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 20-216
-
特异性
- Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.
-
交叉反应
- 人, 小鼠, 大鼠
-
纯化方法
- Protein G Purified
-
免疫原
- Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
-
亚型
- IgG1
-
-
anti-Notch 1 (NOTCH1) antibody (APC)
NOTCH1 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal mN1A APC
anti-Notch 1 (NOTCH1) antibody (PE)NOTCH1 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal mN1A PE
anti-Notch 1 (NOTCH1) (AA 1919-2079) antibodyNOTCH1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) (AA 2101-2300) antibodyNOTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) (Cleaved-Val1754) antibodyNOTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) antibodyKD Validated NOTCH1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB425 unconjugated Recombinant Antibody
anti-Notch 1 (NOTCH1) (AA 20-216) antibodyNOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 unconjugated
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (HRP)NOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 HRP
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (FITC)NOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 FITC
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (PE)NOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 PE
-
-
应用备注
-
- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
-
说明
-
1 μg/ml of ABIN2483199 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Conjugated antibodies should be stored at 4°C
-
-
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
-
别名
- Notch1
-
背景
- Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
-
基因ID
- 18128
-
NCBI登录号
- NP_032740
-
UniProt
- Q01705
-
途径
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
-
