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GFP 抗体 (full length)

This anti-GFP antibody is a 兔 多克隆 antibody detecting GFP in WB, ELISA 和 IHC. Suitable for Aequorea victoria.
产品编号 ABIN2467764
发货至: 中国

Quick Overview for GFP 抗体 (full length) (ABIN2467764)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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  • 1
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克隆类型

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多克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • 抗原表位

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    full length

    纯化方法

    Anti-GFP Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.

    免疫原

    Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
  • 应用备注

    Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Recommended dilutions are as follows, ELISA 1:15,000 - 1:60,000, Western Blot 1:500 - 1:5,000, Immunohistochemistry 1:200 - 1:3,000. (Optimal dilutions should be determined by user).

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01 % (w/v) Sodium Azide, pH 7.2

    注意事项

    Avoid freeze thaw cycles

    储存条件

    -20 °C

    储存方法

    Store vial at -20 C or below prior to opening. Dilute only prior to immediate use. Forextended storage, aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    基因ID

    7011691

    NCBI登录号

    YP_002302326

    UniProt

    P42212
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