HNF4A 抗体 (N-Term)
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Quick Overview for HNF4A 抗体 (N-Term) (ABIN2465821)
抗原
See all HNF4A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody is expected to recognize the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).
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纯化方法
- HNF4A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- HNF4A antibody was raised against a 14 amino acid synthetic peptide near the N-Terminus of HNF4A.
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应用备注
- ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 52 kDa and 48 kDa bands observed in lysates of human hepatocellular carcinoma cell line HepG2 (calculated MW of 52.8 kDa according to NP_000448.3 and 46.6 kDa according to NP_849181.1). Recommended concentration: 0.1-0.3 μg/mL.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20 °C.
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- HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
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别名
- HNF4A
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基因ID
- 3172
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NCBI登录号
- NP_849180, NP_000448, NP_849181
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UniProt
- P41235
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途径
- AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
抗原
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