Western Blot:Dilution 1:1,000 - 1:5,000 IHC(P):Dilution 1:100 - 1:400 IHC(F):Dilution 1:200 - 1:400 Determining optimal working dilutions by titration test.
限制
仅限研究用
状态
Liquid
缓冲液
Ascitic fluid containing 0.03 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
储存条件
-20 °C
储存方法
Trim5? monoclonal antibody can be stored at -20 °C, stable for one year.
抗原
TRIM5
(Tripartite Motif Containing 5 (TRIM5))
别名
Trim5a
背景
TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5alpha, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.