FEN1 抗体
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北京 101111
Quick Overview for FEN1 抗体 (ABIN2462939)
抗原
See all FEN1 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by protein A chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FEN1.
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anti-Flap Structure-Specific Endonuclease 1 (FEN1) (AA 50-380) antibody
FEN1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibodyFEN1 适用: 人 WB, IHC, IF, IP, IHC (p), ICC, PLA 宿主: 兔 Polyclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibodyFEN1 适用: 人, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibodyFEN1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Monoclonal 4D9 unconjugated Recombinant Antibody
anti-Flap Structure-Specific Endonuclease 1 (FEN1) (AA 1-380) antibodyFEN1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibodyFEN1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) (Internal Region) antibodyFEN1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibodyFEN1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibodyFEN1 适用: 人, 小鼠 WB, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Flap Structure-Specific Endonuclease 1 (FEN1) antibodyFEN1 适用: 人, 小鼠 WB, IP 宿主: 小鼠 Monoclonal unconjugated
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应用备注
- FEN1 antibody can be used for detection of FEN1 by ELISA at 1:62500. FEN1 antibody can be used for detection of FEN1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store FEN1 antibody at -20 °C.
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- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
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别名
- FEN1
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背景
- FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
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分子量
- 42 kDa
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基因ID
- 2237
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NCBI登录号
- NP_004102
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UniProt
- P39748
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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