MBD1 抗体
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北京 101111
Quick Overview for MBD1 抗体 (ABIN2460355)
抗原
See all MBD1 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by peptide affinity chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBD1.
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anti-Methyl-CpG Binding Domain Protein 1 (MBD1) antibody
KD Validated MBD1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB405 unconjugated Recombinant Antibody
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 415-508) antibodyMBD1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2C7 unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 415-508) antibodyMBD1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4F6 unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (C-Term) antibodyMBD1 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 1-605) antibodyMBD1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 106-355) antibodyMBD1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 376-405), (C-Term) antibodyMBD1 适用: 人 WB 宿主: 兔 Polyclonal RB2371 unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (C-Term) antibodyMBD1 适用: 人, 大鼠, 犬, Cow, 豚鼠, 马, 小鼠, 兔, 猴, Bat, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (C-Term) antibodyMBD1 适用: 人, 大鼠, 犬, Cow, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (N-Term) antibodyMBD1 适用: 人 WB, ELISA, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
- MBD1 antibody can be used for detection of MBD1 by ELISA at 1:312500. MBD1 antibody can be used for detection of MBD1 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store MBD1 antibody at -20 °C.
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- MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))
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别名
- MBD1
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背景
- MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.
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分子量
- 60 kDa, 55 kDa, 65 kDa, 67 kDa, 61 kDa
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基因ID
- 4152
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NCBI登录号
- NP_056669
抗原
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