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GFP 抗体

Cited in 1 publication. This 大鼠 单克隆 anti-GFP antibody (Clone 1A5) specifically detects GFP in WB, ELISA, IP, IC 和 ChIP-seq. The antibody is reactive with Aequorea victoria samples.
产品编号 ABIN2451988
发货至: 中国
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Quick Overview for GFP 抗体 (ABIN2451988)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

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大鼠

克隆类型

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单克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunochromatography (IC), ChIP DNA-Sequencing (ChIP-seq)

克隆位点

1A5
  • 特异性

    Specific to GFP and GFP-fused proteins

    产品特性

    The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

    纯化方法

    Purified

    过滤

    Sterile filtered

    免疫原

    Recombinant GFP protein

    亚型

    IgG1 kappa
  • 应用备注

    1. Western blotting: ~1 g/mL
    2. Immunoprecipitation
    3. Immunocytochemistry
    4. Chromatin Immunoprecipitation (ChIP)
    5. ELISA

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol

    储存液

    Azide free

    储存条件

    -20 °C/-80 °C

    储存方法

    -20 C (For long term storage: -80 C)
  • SHIMOMURA, JOHNSON, SAIGA: "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea." in: Journal of cellular and comparative physiology, Vol. 59, pp. 223-39 (PubMed).

  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light. In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization.

    UniProt

    P42212
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