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WISP1 抗体 (C-Term)

This 山羊 多克隆 anti-WISP1 antibody specifically detects WISP1 in WB, ELISA, IF 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN238610
发货至: 中国
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中国
北京 101111
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Room 801-803
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Quick Overview for WISP1 抗体 (C-Term) (ABIN238610)

抗原

See all WISP1 抗体
WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This WISP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    WISP1

    序列

    ESYPDFSEIA N

    特异性

    This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

    别名

    CCN4

    背景

    WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1

    基因ID

    8840, 22402, 65154

    NCBI登录号

    NP_003873, NP_543028

    途径

    WNT signaling, Growth Factor Binding
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