WISP1 抗体 (C-Term)
Quick Overview for WISP1 抗体 (C-Term) (ABIN238610)
抗原
See all WISP1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- WISP1
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序列
- ESYPDFSEIA N
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特异性
- This antibody is expected to recognize both reported isoforms (NP_003873.1, NP_543028.1).
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1, NP_543028.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 32-35 kDa band observed in lysates of cell line CaCo-2 and approx. 40 kDa in Human Tonsil lysates, and additionally in a lysate of cell line CaCo-2, which were successfully blocked by incubation with the immunising peptide (calculated
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))
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别名
- CCN4
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背景
- WISP1, WNT1 inducible signaling pathway protein 1, CCN4, WISP1c, WISP1i, WISP1tc, WNT1 induced secreted protein 1, Wnt-1 inducible signaling pathway protein 1, wnt-1 signaling pathway protein 1
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基因ID
- 8840, 22402, 65154
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NCBI登录号
- NP_003873, NP_543028
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途径
- WNT signaling, Growth Factor Binding
抗原
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