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CK1 epsilon 抗体 (C-Term)

This anti-CK1 epsilon antibody is a 山羊 多克隆 antibody detecting CK1 epsilon in WB, ELISA, IHC, IF 和 FACS. Suitable for 人.
产品编号 ABIN238540
发货至: 中国

Quick Overview for CK1 epsilon 抗体 (C-Term) (ABIN238540)

抗原

See all CK1 epsilon (CSNK1E) 抗体
CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This CK1 epsilon antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    CSNK1E

    序列

    PASQTSVPFD HLGK

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

    别名

    CSNK1E

    背景

    CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon

    基因ID

    1454, 27373, 58822

    NCBI登录号

    NP_001276841

    途径

    Hedgehog Signaling, M Phase
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